Detection of monoclonality in intestinal lymphoma with polymerase chain reaction for antigen receptor gene rearrangement analysis to differentiate from enteritis in dogs

The diagnosis of canine intestinal lymphoma by morphological examination is challenging, especially when endoscopic tissue specimens are used. The utility of detection of antigen receptor gene rearrangement by polymerase chain reaction (PARR) in canine lymphoma has been well established, but its use...

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Bibliographic Details
Published in:Veterinary & comparative oncology 2017-03, Vol.15 (1), p.194-207
Main Authors: Ohmura, S., Leipig, M., Schöpper, I., Hergt, F., Weber, K., Rütgen, B. C., Tsujimoto, H., Hermanns, W., Hirschberger, J.
Format: Article
Language:English
Subjects:
alimentary lymphoma
Animals
clonality
Diagnosis, Differential
DNA Primers
Dog Diseases - diagnosis
Dog Diseases - genetics
Dog Diseases - pathology
Dogs
Enteritis - pathology
Enteritis - veterinary
enteropathy
Female
FFPE tissue
Germany
Immunochemistry
immunophenotyping
Intestinal Neoplasms - diagnosis
Intestinal Neoplasms - genetics
Intestinal Neoplasms - pathology
Intestinal Neoplasms - veterinary
Lymphoma - diagnosis
Lymphoma - genetics
Lymphoma - pathology
Lymphoma - veterinary
Male
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Receptors, Antigen, B-Cell - analysis
Receptors, Antigen, T-Cell - analysis
Retrospective Studies
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Summary:The diagnosis of canine intestinal lymphoma by morphological examination is challenging, especially when endoscopic tissue specimens are used. The utility of detection of antigen receptor gene rearrangement by polymerase chain reaction (PARR) in canine lymphoma has been well established, but its usefulness to distinguish enteritis and intestinal lymphoma remains unclear. In this retrospective study we assessed clonality of 29 primary canine intestinal lymphoma, 14 enteritis and 15 healthy control cases by PARR analysis, using formalin‐fixed, paraffin‐embedded full‐thickness tissue specimens. We could detect monoclonal rearrangements in 22 of 29 canine intestinal lymphomas [76%; 95% confidence interval (CI) 56–90%] and polyclonal rearrangements in all of the enteritis and healthy control cases (100%; CI 88–100%). We revealed a predominance of T‐cell phenotype compared to B‐cell phenotype (85%; CI 65–96% and 15%; CI 4–35%, respectively). We showed that PARR analysis contributes to differentiation of canine intestinal lymphoma from enteritis and to phenotyping of lymphomas.
ISSN:1476-5810
1476-5829
DOI:10.1111/vco.12151