Identification of a miRNA signature in neutrophils after traumatic injury

Traumatic injury is the cause of significant mortality and morbidity. The molecular mechanisms underlying traumatic injury logically involve changes in gene expression that may be regulated through microRNAs （miRNAs）. However, the association between miRNA deregulation and traumatic injury is largel...

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Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2013-11, Vol.45 (11), p.938-945
Main Authors: Yang, Jun, Liang, Yong, Han, Huazhong, Qin, Huanlong
Format: Article
Language:English
Subjects:
Adult
Aged
Algorithms
Case-Control Studies
Female
Gene Regulatory Networks
Humans
Male
MicroRNAs - genetics
Middle Aged
miRNA
Neutrophils - metabolism
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Wounds and Injuries - blood
Wounds and Injuries - genetics
嗜中性粒细胞
基因本体论
外伤性
定量RT-PCR
签名
识别
跌打损伤
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Summary:Traumatic injury is the cause of significant mortality and morbidity. The molecular mechanisms underlying traumatic injury logically involve changes in gene expression that may be regulated through microRNAs （miRNAs）. However, the association between miRNA deregulation and traumatic injury is largely unknown. The purpose of the present study is to address this issue. In this study, we used microarray profiling to evaluate the differential expressions of miRNAs in neutrophils obtained from patients with major trauma （injury severity scores 〉16）, relative to healthy individuals. This neutrophilic miRNA signature was further validated using quantitative real-time reverse transcription-polymer- ase chain reaction （qRT-PCR）. Genes and signaling path- ways related to trauma-induced deregulated miRNAs were investigated in silico using the ontology-based and network mapping algorithms of Gene Ontology and Kyoto Encyclopedia of Genes or Genomes. Results showed that 13 miRNAs in neutrophils of major trauma patients were significantly and differentially expressed compared with the miRNA profiles of healthy controls. The results of qRT- PCR and in silico analysis revealed that miR-23a-5p, miR-30e-3p, mil~-223-5p, miR-3945, miR-155-5p, and miR- 150-5p were likely participants in the traumatic pathogen- esis of these patients. In conclusion, neutrophils associated with traumatic injury were found to have a unique miRNA signature. Changes in signaling pathways due to deregulated miRNAs may be involved in the pathological processes of traumatic injury.
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmt100