Thyroid hormone regulates oxidative phosphorylation in the cerebral cortex and striatum of neonatal rats

We have previously shown that thyroid hormone (T3) regulates mitochondrial gene expression, morphology and transmembrane potential in the developing brain. Here, we have analysed the effect of thyroid hormone on mitochondrial function in different brain regions. For this purpose we have determined,...

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Bibliographic Details
Published in:Journal of neurochemistry 2001-09, Vol.78 (5), p.1054-1063
Main Authors: Martinez, Bienvenida, Del Hoyo, Pilar, Martin, Miguel Angel, Arenas, Joaquín, Perez‐Castillo, Ana
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Animals
Animals, Newborn
Antithyroid Agents
Biochemistry and metabolism
Biological and medical sciences
brain
Carrier Proteins
Central nervous system
Cerebral Cortex - growth & development
Cerebral Cortex - metabolism
Corpus Striatum - growth & development
Corpus Striatum - metabolism
development
Electron Transport Complex I
Electron Transport Complex II
Electron Transport Complex III - metabolism
Electron Transport Complex IV - metabolism
Enzyme Activation - physiology
Female
Fundamental and applied biological sciences. Psychology
gene expression
Hypothyroidism - chemically induced
Hypothyroidism - metabolism
Membrane Proteins - metabolism
Methimazole
mitochondria
Mitochondria - drug effects
Mitochondria - enzymology
Multienzyme Complexes - metabolism
NADH, NADPH Oxidoreductases - metabolism
oxidative phosphorylation
Oxidative Phosphorylation - drug effects
Oxidoreductases - metabolism
Pregnancy
Prenatal Exposure Delayed Effects
Rats
Rats, Wistar
RNA - analysis
RNA, Mitochondrial
Succinate Dehydrogenase - metabolism
thyroid hormone
Triiodothyronine - pharmacology
Vertebrates: nervous system and sense organs
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Summary:We have previously shown that thyroid hormone (T3) regulates mitochondrial gene expression, morphology and transmembrane potential in the developing brain. Here, we have analysed the effect of thyroid hormone on mitochondrial function in different brain regions. For this purpose we have determined, in control, hypothyroid and T3‐treated hypothyroid neonatal rats, the rate of oxidative phosphorylation in isolated mitochondria and the activity of the respiratory complexes in tissue homogenates. Our results showed a decrease in oxidative phosphorylation rate (only in the presence of NADH‐generating substrates) and mitochondrial complexes I and III activity in the cerebral cortex and striatum of hypothyroid neonates, but not in the other areas analysed (hippocampus, cerebellum, thalamus, mid brain and brain stem). In parallel with mitochondrial activity, the levels of mitochondrially encoded transcripts were decreased only in the cerebral cortex and striatum of hypothyroid rats. The administration of T3 corrected all these parameters. In summary, this study showed a down‐regulation of mitochondrial gene expression accompanied by a decrease in mitochondrial activity in the cerebral cortex and striatum of developing hypothyroid neonatal rats.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.2001.00487.x