Enhanced effect of κ-carrageenan on TNBS-induced inflammation in mice

As a sulfated polysaccharide, carrageenan has been widely used as common food additive. In the present study, we investigated the effects of κ-carrageenan on TNBS-induced gut inflammation in mice. BALB/c mice were pretreated with κ-carrageenan for 14days prior to the administration of TNBS. Our resu...

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Bibliographic Details
Published in:International immunopharmacology 2016-10, Vol.39, p.218-228
Main Authors: Wei, Wu, Feng, Wang, Xin, Gao, Tingting, Niu, Zhanghe, Zhen, Haimin, Chen, Xiaojun, Yan
Format: Article
Language:English
Subjects:
Aggravate
Animals
Carrageenan - immunology
CD4-Positive T-Lymphocytes - immunology
Colitis - chemically induced
Colitis - immunology
Colon - immunology
Crohn Disease - immunology
Cytokines - metabolism
Disease Models, Animal
Female
Humans
Intestinal inflammation
Male
Mice
NF-kappa B - metabolism
TNBS
Toll-Like Receptor 4 - metabolism
Trinitrobenzenesulfonic Acid
κ-Carrageenan
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Summary:As a sulfated polysaccharide, carrageenan has been widely used as common food additive. In the present study, we investigated the effects of κ-carrageenan on TNBS-induced gut inflammation in mice. BALB/c mice were pretreated with κ-carrageenan for 14days prior to the administration of TNBS. Our results showed that κ-carrageenan pretreatment aggravated the loss of body weight and further increased the mortality rate. Histological and morphological analyses revealed that the TNBS-induced colonic inflammation was deteriorated by the κ-carrageenan administration. The ratio of CD4+CD25+CD127dim/CD4+ of the κ-carrageenan+TNBS groups was significantly lower than that of the TNBS group. The expression of IL-2, TNF-α and IL-6 was significantly increased, whereas the expression of IL-10 was significantly decreased in the κ-carrageenan+TNBS groups. In addition, κ-carrageenan, together with TNBS, decreased the enzyme activity of SOD and GSH-px and up-regulated the expression of TLR4, NF-κB, p-ERK, p-JNK, p-Jun., IL-8 and MDA in the colonic mucosa. κ-Carrageenan aggravated the TNBS-induced intestinal inflammation, and such an effect could be associated with the oxidative stress and activation of TLR4-NF-κB and MAPK/ERK1/2 pathway. [Display omitted] •The synergistic effect of κ-carrageenan linked to the induction of an imbalance in the expression of intestinal inflammatory cytokines.•κ-carrageenan significantly aggravated TNBS-induced inflammation in BALB/c mice.•κ-carrageenan might serve as a potential inflammatory agent to magnify the existing intestinal inflammation under the intestinal inflammatory state.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2016.07.031