Monitoring of chlorsulfuron in biological fluids and water samples by molecular fluorescence using rhodamine B as fluorophore

A new simple methodology is proposed for chlorsufuron (CS) traces quantification based upon enhancement of rhodamine B (RhB) fluorescent signal. Experimental variables that influence fluorimetric sensitivity have been studied and optimized. The zeroth order regression calibration was linear from 0.8...

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Bibliographic Details
Published in:Talanta (Oxford) 2016-11, Vol.160, p.431-436
Main Authors: Alesso, Magdalena, Escudero, Luis A., Talio, María Carolina, Fernández, Liliana P.
Format: Article
Language:English
Subjects:
Biological and water samples
Chlorsulfuron monitoring
Drinking Water - analysis
Environmental Pollutants - analysis
Environmental Pollutants - blood
Environmental Pollutants - chemistry
Environmental Pollutants - urine
Female
Fluorescence
Fluorescent Dyes - chemistry
Groundwater - analysis
Humans
Milk, Human - chemistry
Molecular fluorescence
Pesticides - analysis
Pesticides - blood
Pesticides - chemistry
Pesticides - urine
Plasma - chemistry
Rhodamine B
Rhodamines - chemistry
Serum - chemistry
Spectrometry, Fluorescence
Sulfonamides - analysis
Sulfonamides - blood
Sulfonamides - chemistry
Sulfonamides - urine
Triazines - analysis
Triazines - blood
Triazines - chemistry
Triazines - urine
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Summary:A new simple methodology is proposed for chlorsufuron (CS) traces quantification based upon enhancement of rhodamine B (RhB) fluorescent signal. Experimental variables that influence fluorimetric sensitivity have been studied and optimized. The zeroth order regression calibration was linear from 0.866 to 35.800µgL−1 CS, with a correlation coefficient of 0.99. At optimal experimental conditions, a limit of detection of 0.259µgL−1 and a limit of quantification of 0.866µgL−1 were obtained. The method showed good sensitivity and adequate selectivity and was applied to the determination of trace amounts of CS in plasma, serum and water samples with satisfactory results analyzed by ANOVA test. The proposed methodology represents an alternative to traditional chromatographic techniques for CS monitoring in complex samples, using an accessible instrument in control laboratories. [Display omitted] •An alternative to traditional chlorsufuron (CS) monitoring methods is proposed.•An enhancement in fluorescent signal of RhB was evidenced in the presence of CS.•The methodology was applied to biological fluids and water samples.•The use of non-polluting solvents preserves the analyst and the environment.•This work represents an important contribution at green chemistry.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2016.07.053