Biocompatible cephalosporin-hydroxyapatite-poly(lactic-co-glycolic acid)-coatings fabricated by MAPLE technique for the prevention of bone implant associated infections

•HAp/PLGA thin coatings by Matrix Assisted Pulsed Laser Evaporation.•Anti-adherent coating on medical surfaces against S. aureus and P. aeruginosa colonization.•Coatings with potential applications in implant osseointegration. In this study we aimed to obtain functionalized thin films based on hydro...

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Published in:Applied surface science 2016-06, Vol.374, p.387-396
Main Authors: Rădulescu, Dragoş, Grumezescu, Valentina, Andronescu, Ecaterina, Holban, Alina Maria, Grumezescu, Alexandru Mihai, Socol, Gabriel, Oprea, Alexandra Elena, Rădulescu, Marius, Surdu, Adrian, Trusca, Roxana, Rădulescu, Radu, Chifiriuc, Mariana Carmen, Stan, Miruna S., Constanda, Sabrina, Dinischiotu, Anca
Format: Article
Language:English
Subjects:
Antibiotics
Biofilms
Bones
Hydroxyapatite
Implants
Laser processing
MAPLE
Microorganisms
Osseointegration
PLGA
Surgical implants
Thin films
Transmission electron microscopy
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Summary:•HAp/PLGA thin coatings by Matrix Assisted Pulsed Laser Evaporation.•Anti-adherent coating on medical surfaces against S. aureus and P. aeruginosa colonization.•Coatings with potential applications in implant osseointegration. In this study we aimed to obtain functionalized thin films based on hydroxyapatite/poly(lactic-co-glycolic acid) (HAp/PLGA) containing ceftriaxone/cefuroxime antibiotics (ATBs) deposited by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The prepared thin films were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-Ray diffraction (XRD), selected area electron diffraction (SAED), and infra red (IR) analysis. HAp/PLGA/ATBs thin films sustained the growth of human osteoblasts, proving their good biocompatibility. The microscopic evaluation and the culture-based quantitative assay of the E. coli biofilm development showed that the thin films inhibited the initial step of microbial attachment as well as the subsequent colonization and biofilm development on the respective surfaces. This study demonstrates that MAPLE technique could represent an appealing technique for the fabrication of antibiotics-containing polymeric implant coatings. The bioevaluation results recommend this type of surfaces for the prevention of bone implant microbial contamination and for the enhanced stimulation of the implant osseointegration process.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2016.02.072