Fabrication of a third-generation glucose biosensor using graphene-polyethyleneimine-gold nanoparticles hybrid

[Display omitted] •A novel third generation biosensor was fabricated for glucose measurement.•A hybrid was prepared using graphene, polyethyleneimine and gold nanoparticles.•Glucose oxidase was immobilized on GNS-PEI-AuNPs modified Au electrode.•Direct electron transfer property was observed for the...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2016-09, Vol.232, p.454-461
Main Authors: Rafighi, Parvin, Tavahodi, Mojtaba, Haghighi, Behzad
Format: Article
Language:English
Subjects:
Adenines
Biosensors
Crosslinking
Direct electron transfer
Electrochemical biosensor
Electrodes
Electron transfer
Glucose
Glucose biosensor
Nanoparticles
Separation
Third-generation biosensor
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Summary:[Display omitted] •A novel third generation biosensor was fabricated for glucose measurement.•A hybrid was prepared using graphene, polyethyleneimine and gold nanoparticles.•Glucose oxidase was immobilized on GNS-PEI-AuNPs modified Au electrode.•Direct electron transfer property was observed for the immobilized GOx.•The immobilized GOx catalyzed oxidation of glucose in the absence of oxygen. The present study reports a novel approach for the fabrication of a third generation glucose biosensor based on the immobilization of glucose oxidase (GOx) on graphene-polyethyleneimine-gold nanoparticles hybrid (GNS-PEI-AuNPs) using glutaraldehyde as cross-linking reagent. A pair of well-defined redox peaks with the formal potential and peak separation value of −0.38V and 60mV was observed for the immobilized GOx on a gold electrode modified with GNS-PEI-AuNPs hybrid. The heterogeneous electron transfer rate (ks) for the redox center of the immobilized GOx, flavin adenine dinucleotide (FAD), was found to be 5.4s−1. An increase for the anodic peak current and a decrease for the cathodic peak current were recorded for the immobilized GOx on the modified electrode in the presence of glucose and the absence of oxygen. The obtained results confirmed that the prepared modified electrode presented bioelectrocatalytic activity towards glucose through a direct electron transfer (DET) mechanism. The response of the fabricated glucose biosensor was linear towards glucose in the concentration range between 1 and 100μM with the sensitivity about 93μAmM−1cm−2. The limit of detection (LOD) for glucose sensing was estimated to be 0.32μM (S/N=3).
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.03.147