Real-time label-free quantitative fluorescence microscopy-based detection of ATP using a tunable fluorescent nano-aptasensor platform

Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological sample...

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Bibliographic Details
Published in:Nanoscale 2015-12, Vol.7 (46), p.19663-19672
Main Authors: Shrivastava, Sajal, Sohn, Il-Yung, Son, Young-Min, Lee, Won-Il, Lee, Nae-Eung
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Adenosine Triphosphate - analysis
Biomolecules
Biosensing Techniques - methods
Fluorescence
Graphene
Graphite - chemistry
Nanoparticles - chemistry
Nanostructure
Platforms
Real time
Strategy
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Summary:Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological samples. Here we introduce an ensemble strategy for a real-time label-free fluorescent graphene (Gr) aptasensor platform. This platform employs aptamer length-dependent tunability, thus enabling the reagentless quantitative detection of biomolecules through computational processing coupled with real-time fluorescence imaging data. We demonstrate that this strategy effectively delivers dose-dependent quantitative readouts of adenosine triphosphate (ATP) concentration on chemical vapor deposited (CVD) Gr and reduced graphene oxide (rGO) surfaces, thereby providing cytotoxicity assessment. Compared with conventional fluorescence spectrometry methods, our highly efficient, universally applicable, and rational approach will facilitate broader implementation of imaging-based biosensing platforms for the quantitative evaluation of a range of target molecules.
ISSN:2040-3364
2040-3372
DOI:10.1039/c5nr05839b