Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning

In this study, enzyme-assisted three-phase partitioning (EATPP) was used to extract oil from flaxseed. The whole procedure is composed of two parts: the enzymolysis procedure in which the flaxseed was hydrolyzed using an enzyme solution (the influencing parameters such as the type and concentration...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2016-08, Vol.179 (8), p.1325-1335
Main Authors: Tan, Zhi-jian, Yang, Zi-zhen, Yi, Yong-jian, Wang, Hong-ying, Zhou, Wan-lai, Li, Fen-fang, Wang, Chao-yun
Format: Article
Language:English
Subjects:
Ammonium Sulfate - chemistry
Biochemistry
Biotechnology
Chemical Fractionation - methods
Chemistry
Chemistry and Materials Science
Enzymes
Extraction processes
Flax
Flax - chemistry
Gas Chromatography-Mass Spectrometry
Kinetics
Linseed Oil - isolation & purification
Linum usitatissimum
Oils & fats
Peptide Hydrolases - metabolism
Polygalacturonase - metabolism
Temperature
tert-Butyl Alcohol - chemistry
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Summary:In this study, enzyme-assisted three-phase partitioning (EATPP) was used to extract oil from flaxseed. The whole procedure is composed of two parts: the enzymolysis procedure in which the flaxseed was hydrolyzed using an enzyme solution (the influencing parameters such as the type and concentration of enzyme, temperature, and pH were optimized) and three-phase partitioning (TPP), which was conducted by adding salt and t -butanol to the crude flaxseed slurry, resulting in the extraction of flaxseed oil into alcohol-rich upper phase. The concentration of t -butanol, concentration of salt, and the temperature were optimized to maximize the extraction yield. Under optimized conditions of a 49.29 % t- butanol concentration, 30.43 % ammonium sulfate concentration, and 35 °C extraction temperature, a maximum extraction yield of 71.68 % was obtained. This simple and effective EATPP can be used to achieve high extraction yields and oil quality, and thus, it is potential for large-scale oil production.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-016-2068-x