Duodenal‐jejunal bypass surgery induces hepatic lipidomic alterations associated with ameliorated hepatic steatosis in mice

Objective Bariatric surgery induces weight loss and improvement of insulin resistance; one aspect of both is an amelioration of hepatic steatosis. This study was undertaken to assess the changes in the hepatic lipidome after duodenal‐jejunal bypass (DJB) surgery. Methods A DJB surgical model was dev...

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Published in:Obesity (Silver Spring, Md.) Md.), 2016-09, Vol.24 (9), p.1938-1945
Main Authors: Shang, Jin, Castro‐Perez, Jose M., Shen, Xiaolan, Zhu, Yonghua, Liu, Haiying, Qian, Ying, Previs, Stephen, Howard, Andrew D., Erion, Mark, Kelley, David E., Wang, Liangsu
Format: Article
Language:English
Subjects:
Animals
Bariatric Surgery
Blood Glucose - metabolism
Duodenum - surgery
Fatty acids
Fatty Liver - metabolism
Fatty Liver - surgery
Gastric Bypass - methods
Insulin Resistance
Jejunum - surgery
Kinases
Liver
Male
Mice
Rodents
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Summary:Objective Bariatric surgery induces weight loss and improvement of insulin resistance; one aspect of both is an amelioration of hepatic steatosis. This study was undertaken to assess the changes in the hepatic lipidome after duodenal‐jejunal bypass (DJB) surgery. Methods A DJB surgical model was developed and characterized in diet‐induced obese mice. In comparison with sham‐operated mice, an unbiased lipidomic profiling of hepatic lipids was performed together with measurements of gene expression within key pathways of hepatic lipid metabolism. Results In the liver of DJB mice, a dramatic reduction (by 77%) in hepatic triacylglycerols was observed. Global lipidomic profiling identified marked decreases of triacylglycerols comprised of medium length fatty acids and with low double bond content. Specific diacylglycerol species were also among the most dramatic decreases in hepatic lipids, whereas lysophosphatidic acids and phosphatidic acids were increased. Expression of fatty acid transporter and lipogenic genes was down‐regulated. Conclusions From in‐depth analysis of hepatic lipid composition, specific lipid intermediates were identified that are preferentially changed following DJB surgery. These changes were most likely due to DJB‐induced weight loss, and only further studies will be able to distinguish weight loss‐dependent from weight loss‐independent changes.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.21583