Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green

Background and Objectives The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin. Methods Fresh‐fixed LNs in c...

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Bibliographic Details
Published in:Journal of surgical oncology 2016-09, Vol.114 (3), p.348-353
Main Authors: Liberale, Gabriel, Galdon, Maria Gomez, Moreau, Michel, Vankerckhove, Sophie, El Nakadi, Issam, Larsimont, Denis, Donckier, Vincent, Bourgeois, Pierre
Format: Article
Language:English
Subjects:
Aged
colorectal neoplasms
Colorectal Neoplasms - pathology
Colorectal Neoplasms - surgery
ex vivo
Female
fluorescence imaging
Fluorescent Dyes
Humans
Indocyanine Green
Lymph Node Excision
lymph nodes
Lymph Nodes - diagnostic imaging
Male
metastatic
Middle Aged
Optical Imaging
Peritoneal Neoplasms - diagnostic imaging
Peritoneal Neoplasms - secondary
Peritoneal Neoplasms - surgery
Sensitivity and Specificity
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Summary:Background and Objectives The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin. Methods Fresh‐fixed LNs in cassettes and/or paraffin‐embedded LNs of patients included in a study that evaluated the role of ICG in the detection of peritoneal metastases of colorectal origin (Protocol NCT‐01995591) were further explored with a dedicated near‐infrared camera system for their fluorescence. An IV injection of ICG was delivered intraoperatively at 0.25 mg/kg. Signal to background ratios (SBRs) were calculated. Results LNs on operative specimens were evaluated for 12 patients (5 males, 7 females). A total of 182 LNs were analyzed. The mean LN number per patient was 15.2 (median: 15.5; range 3–22). SBRs of mLNs were significantly more fluorescent than benign LNs, 1.41 versus 1.04 arbitrary units (P 20 mm2) (P 
ISSN:0022-4790
1096-9098
DOI:10.1002/jso.24318