HnRNP K contributes to drug resistance in acute myeloid leukaemia through the regulation of autophagy

Abstract The goal of this study was to explore the role of hnRNP K in drug resistance through the regulation of autophagy in acute myeloid leukaemia. First, we verified the connection between the expression level of hnRNP K and the level of drug resistance in myeloid leukaemia using fluorescence qua...

Full description

Saved in:
Bibliographic Details
Published in:Experimental hematology 2016-09, Vol.44 (9), p.850-856
Main Authors: Zhang, JinFang, Liu, XiaoLi, Lin, YuDeng, Li, YuLing, Pan, JianWei, Zong, Sa, Li, YongKang, Zhou, Yang
Format: Article
Language:English
Subjects:
Adolescent
Advanced Basic Science
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Autophagy - drug effects
Autophagy - genetics
Cell Line, Tumor
Child
Child, Preschool
Drug Resistance, Neoplasm - genetics
Female
Gene Expression
Genotype
Hematology, Oncology and Palliative Medicine
Heterogeneous-Nuclear Ribonucleoprotein K - genetics
Humans
Leukemia, Myeloid, Acute - diagnosis
Leukemia, Myeloid, Acute - drug therapy
Leukemia, Myeloid, Acute - genetics
Leukemia, Myeloid, Acute - mortality
Male
Microtubule-Associated Proteins - genetics
RNA Interference
RNA, Small Interfering - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The goal of this study was to explore the role of hnRNP K in drug resistance through the regulation of autophagy in acute myeloid leukaemia. First, we verified the connection between the expression level of hnRNP K and the level of drug resistance in myeloid leukaemia using fluorescence quantitative PCR. Then, we used western blotting to determine the expression level of autophagy related proteins LC3Ⅰ/Ⅱ following the modulation of hnRNP K by RNA interference. Finally, an analysis of adriamycin drug sensitivity was conducted before and after the modulation of hnRNP K expression. hnRNP K and LC3Ⅰ/Ⅱ were significantly overexpressed in the bone marrow of nonremission patients and in drug resistant cell lines, however, the expression of LC3Ⅰ/Ⅱ was decreased when the expression of hnRNP K was reduced, and drug sensitivity to adriamycin could be restored. hnRNP K may be involved in the development of adriamycin resistance in acute myeloid leukaemia through the regulation of autophagy.
ISSN:0301-472X
1873-2399
DOI:10.1016/j.exphem.2016.04.014