Are embryonic developing modes determinant in the acquisition and levels of photoprotective compounds in slipper limpets of the Crepipatella genus?

The type of embryonic development (mixed and direct) and its influence on the accumulation and translocation of photoprotective compounds from the mother to the encapsulated embryo was studied in the intertidal gastropods Crepipatella peruviana and Crepipatella dilatata during their reproductive pea...

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Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2016-09, Vol.162, p.511-518
Main Authors: Paredes-Molina, F.J., Cubillos, V.M., Montory, J.A., Andrade-Villagrán, P.A.
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Animals
Carotenoids
Carotenoids - metabolism
Development mode
Dose-Response Relationship, Radiation
Embryo, Nonmammalian - metabolism
Embryo, Nonmammalian - radiation effects
Embryos
Female
Gastropoda - embryology
Gastropoda - metabolism
Gastropoda - radiation effects
Incubation
Mycosporine-like amino acids
Ontogeny
Ultraviolet Rays - adverse effects
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Summary:The type of embryonic development (mixed and direct) and its influence on the accumulation and translocation of photoprotective compounds from the mother to the encapsulated embryo was studied in the intertidal gastropods Crepipatella peruviana and Crepipatella dilatata during their reproductive peak. HPLC/MS was used to determine type and levels of sunscreen compounds (total carotenoids; TC/and mycosporine-like amino acid; MAA) in brooding females, capsule walls and early and pre-hatching embryos of both species. Photoprotective compounds were only quantified in nurse eggs of C. dilatata. Our results indicate that females of both species can accumulate TC and MAA at different levels, and they are able to transfer them selectively to capsule walls, embryos and nurse eggs. Palythine-serine (MW=244Da; λmax=320nm) and MAA-330 (MW=234Da; λmax=330nm) constitute total MAA pool in brooding females, whereas brooded embryos incorporate palythine (MW=244Da; λmax=320nm) to the MAA pool. Although TC was transferred from the mother to the embryo through the yolk in both species, MAA trespass showed differences. Females of C. peruviana transfer MAA to their embryos through the embryonic yolk; C. dilatata can transfer MAA only through their nurse eggs, which are consumed by embryos during the terminal stages of intracapsular development. Differences between mixed and direct embryonic development, as well as environmental UV-R levels, which the recently hatched larvae and juveniles of C. peruviana and C. dilatata are exposed to, would determine levels of sunscreen compounds in each species. Higher TC and MAA levels in pre-hatching larvae of C. peruviana compared to C. dilatata, indicate a necessity of C. peruviana for protection against UV-R radiation during approximately 15days when their veliger larvae remain in the water column before metamorphosis is complete. Conversely, low photoprotective levels in pre-hatching juveniles of C. dilatata could be related to low UV-R exposure levels due to the direct incorporation to the benthos and the presence of a protective shell. •Embryonic development mode determines acquisition and levels of photoprotective compounds (PC).•PC are selectively transferred from adults to their embryos.•Only yolk provides PC in encapsulated larvae under a mixed developmental mode.•Nurse eggs are the only source of PC in embryos developed directly.•Mixed development embryos need greater PC compared with embryos developed directly.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2016.07.013