gsdf is a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia

SUMMARY Gonadal soma‐derived factor (gsdf) is critical for testicular differentiation in teleosts, yet detailed analysis of Gsdf on testicular differentiation is lacking. In the present study, we knocked out tilapia gsdf using CRISPR/Cas9. F0 gsdf‐deficient XY fish with high mutation rate (≥58%) dev...

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Bibliographic Details
Published in:Molecular reproduction and development 2016-06, Vol.83 (6), p.497-508
Main Authors: Jiang, Dong-Neng, Yang, Hui-Hui, Li, Ming-Hui, Shi, Hong-Juan, Zhang, Xian-Bo, Wang, De-Shou
Format: Article
Language:English
Subjects:
Animals
Fish Proteins - genetics
Fish Proteins - metabolism
Male
Oreochromis niloticus
Sex Determination Processes - physiology
Teleostei
Testis - embryology
Tilapia
Tilapia - embryology
Tilapia - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Transforming Growth Factor beta - genetics
Transforming Growth Factor beta - metabolism
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Summary:SUMMARY Gonadal soma‐derived factor (gsdf) is critical for testicular differentiation in teleosts, yet detailed analysis of Gsdf on testicular differentiation is lacking. In the present study, we knocked out tilapia gsdf using CRISPR/Cas9. F0 gsdf‐deficient XY fish with high mutation rate (≥58%) developed as intersex, with ovotestes 90 days after hatching (dah), and become completely sex‐reversed with ovaries at 180 and 240 dah. Those individuals with a low mutation rate (
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.22642