Performance evaluation of four rapid antigen tests for the detection of Respiratory syncytial virus

Rapid identification of Respiratory syncytial virus (RSV) is important in the management of infected patients. Rapid diagnostic tests (RDT) are widely used for this purpose. This study aimed to evaluate the clinical performance of four RSV antigen tests including the BinaxNow RSV Card test, SD Bioli...

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Bibliographic Details
Published in:Journal of medical virology 2016-10, Vol.88 (10), p.1720-1724
Main Authors: Jung, Bo Kyeung, Choi, Sung Hyuk, Lee, Jong Han, Lee, JungHwa, Lim, Chae Seung
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Antigens
Antigens, Viral - analysis
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Medical tests
Middle Aged
Nasopharynx - virology
Performance evaluation
rapid antigen test
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction
Respiratory syncytial virus
Respiratory Syncytial Virus Infections - diagnosis
Respiratory Syncytial Virus, Human - immunology
Respiratory Syncytial Virus, Human - isolation & purification
Respiratory Tract Infections - diagnosis
Respiratory Tract Infections - virology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
RT-PCR
sensitivity
Sensitivity and Specificity
Serologic Tests - standards
specificity
Virology
Young Adult
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Summary:Rapid identification of Respiratory syncytial virus (RSV) is important in the management of infected patients. Rapid diagnostic tests (RDT) are widely used for this purpose. This study aimed to evaluate the clinical performance of four RSV antigen tests including the BinaxNow RSV Card test, SD Bioline RSV test, BD Veritor RSV test, and Humasis RSV antigen test in comparison with real‐time RT–PCR as the reference method. Nasopharyngeal swabs were collected from 280 patients with symptoms of lower respiratory tract infection and stored at −80°C. All swabs were tested for RSV using four rapid antigen tests and real time RT–PCR. The sensitivity of the BinaxNow RSV Card test, SD Bioline RSV test, BD Veritor RSV test, and Humasis RSV Antigen tests were 62.5%, 61.3%, 65.0%, and 67.5% for RSV A, and 61.3%, 65.0%, 61.3%, and 67.5% for RSV B compared to real time RT–PCR, respectively. The specificity of BD Veritor RSV test was 95.8% and those of the other three RDTs was 100%. Commercial RSV antigen detection assays are useful tools for the rapid diagnosis of RSV infection. However, confirmatory testing is always recommended. J. Med. Virol. 88:1720–1724, 2016. © 2016 Wiley Periodicals, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.24522