Melatonin delivery by nanocapsules during in vitro bovine oocyte maturation decreased the reactive oxygen species of oocytes and embryos

[Display omitted] •Supplementation of Mel-LNC during in vitro Maturation increases cleavage and blastocyst rates.•Decrease of ROS levels and apoptotic cell number/blastocyst are found at Mel-LNC group.•Mel-LNC upregulates GPX1 and SOD2 and downregulates CASP3 and BAX genes.•Mel-LNC is able to cross...

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Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2016-08, Vol.63, p.70-81
Main Authors: Remião, Mariana Härter, Lucas, Caroline Gomes, Domingues, William Borges, Silveira, Tony, Barther, Nathaniele Nebel, Komninou, Eliza Rossi, Basso, Andrea Cristina, Jornada, Denise Soledade, Beck, Ruy Carlos Ruver, Pohlmann, Adriana Raffin, Junior, Antonio Sérgio Varela, Seixas, Fabiana Kömmling, Campos, Vinicius Farias, Guterres, Silvia Stanisçuaski, Collares, Tiago
Format: Article
Language:English
Subjects:
Animals
Antioxidant
Antioxidants - administration & dosage
Apoptosis - drug effects
bcl-2-Associated X Protein - genetics
Bovine oocyte
Caspase 3 - genetics
Cattle
Cell Differentiation - drug effects
Embryo, Mammalian - drug effects
Embryonic Development - drug effects
Female
Fertilization in Vitro
Gene Expression Regulation, Developmental
Glutathione - metabolism
Glutathione Peroxidase - genetics
In vitro maturation
Lipid-core
Melatonin
Melatonin - administration & dosage
Nanocapsules
Nanocapsules - administration & dosage
Nanotechnology
Oocytes - drug effects
Oocytes - physiology
Reactive Oxygen Species - metabolism
Superoxide Dismutase - genetics
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Summary:[Display omitted] •Supplementation of Mel-LNC during in vitro Maturation increases cleavage and blastocyst rates.•Decrease of ROS levels and apoptotic cell number/blastocyst are found at Mel-LNC group.•Mel-LNC upregulates GPX1 and SOD2 and downregulates CASP3 and BAX genes.•Mel-LNC is able to cross zona pellucida in oocytes Maturation. In this work, a promising approach to increase the advantageous properties of melatonin through its encapsulation into lipid-core nanocapsules (LNC) was examined. Oocytes were treated during in vitro maturation with non-encapsulated melatonin (Mel), melatonin-loaded lipid-core nanocapsules (Mel-LNC), and unloaded LNC. Cytotoxicity, meiotic maturation rate, development to the blastocyst stage, reactive oxygen species (ROS) and glutathione levels, mean cell number and apoptotic cell/blastocyst, and mRNA quantification were evaluated. Both Mel and Mel-LNC enhanced in vitro embryo production, however, Mel-LNC proved to be more effective at decreasing ROS levels and the apoptotic cell number/blastocyst, increasing the cleavage and blastocyst rates, up-regulating the GPX1 and SOD2 genes, and down-regulating the CASP3 and BAX genes. Mel-LNC could penetrate into oocytes and remain inside the cells until they reach the blastocyst stage. In conclusion, when melatonin was encapsulated in LNC and applied during in vitro oocyte maturation, some quality aspects of the blastocysts were improved.
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2016.05.016