Adhesion and Fusion of Muscle Cells Are Promoted by Filopodia

Indirect flight muscles (IFMs) in Drosophila are generated during pupariation by fusion of hundreds of myoblasts with larval muscle templates (myotubes). Live observation of these muscles during the fusion process revealed multiple long actin-based protrusions that emanate from the myotube surface a...

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Bibliographic Details
Published in:Developmental cell 2016-08, Vol.38 (3), p.291-304
Main Authors: Segal, Dagan, Dhanyasi, Nagaraju, Schejter, Eyal D., Shilo, Ben-Zion
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Cell Adhesion - physiology
Cell Adhesion Molecules - metabolism
Cell Differentiation
Cell Fusion
Cell Membrane - metabolism
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Male
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - physiology
Myoblasts - cytology
Myoblasts - physiology
Pseudopodia - physiology
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Summary:Indirect flight muscles (IFMs) in Drosophila are generated during pupariation by fusion of hundreds of myoblasts with larval muscle templates (myotubes). Live observation of these muscles during the fusion process revealed multiple long actin-based protrusions that emanate from the myotube surface and require Enabled and IRSp53 for their generation and maintenance. Fusion is blocked when formation of these filopodia is compromised. While filopodia are not required for the signaling process underlying critical myoblast cell-fate changes prior to fusion, myotube-myoblast adhesion appears to be filopodia dependent. Without filopodia, close apposition between the cell membranes is not achieved, the cell-adhesion molecule Duf is not recruited to the myotube surface, and adhesion-dependent actin foci do not form. We therefore propose that the filopodia are necessary to prime the heterotypic adhesion process between the two cell types, possibly by recruiting the cell-adhesion molecule Sns to discrete patches on the myoblast cell surface. [Display omitted] •Myotubes project multiple actin-based filopodia•Ena and IRSp53 participate in formation of the filopodia•Filopodia are essential for myoblast-myotube fusion•Contact with filopodia primes myoblasts for productive adhesion with the myotube Indirect Drosophila flight muscles are generated by fusion of myoblasts with larval muscle templates (myotubes). Segal et al. identify through live observation multiple long actin-based protrusions emanating from the myotube surface. Disruption of these filopodia hinders myoblast fusion due to defective priming of heterotypic adhesion between the two cell types.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2016.07.010