Impact of e-cigarette refill liquid with or without nicotine on liver function in adult rats

This study was conducted to evaluate the effects of e-cigarette refill liquid administration alone or with nicotine on the antioxidant defense status, functional and histopathological changes in adult rat liver tissue. For this purpose, 32 rats were treated for 28 days as follows: control group was...

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Bibliographic Details
Published in:Toxicology mechanisms and methods 2016-07, Vol.26 (6), p.433-440
Main Authors: El Golli, Narges, Jrad-Lamine, Aicha, Neffati, Hajira, Rahali, Dalila, Dallagi, Yosra, Dkhili, Houssem, Ba, Nathalie, El May, Michele V., El Fazaa, Saloua
Format: Article
Language:English
Subjects:
Animals
Biomarkers - blood
E-liquid
Electronic Nicotine Delivery Systems - adverse effects
histopathology
Injections, Intraperitoneal
Lipid Peroxidation - drug effects
Liver - drug effects
Liver - enzymology
Liver - pathology
Liver Function Tests
liver injury
Male
nicotine
Nicotine - administration & dosage
Nicotine - toxicity
Organ Size - drug effects
oxidative stress
Oxidative Stress - drug effects
Rats, Wistar
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Summary:This study was conducted to evaluate the effects of e-cigarette refill liquid administration alone or with nicotine on the antioxidant defense status, functional and histopathological changes in adult rat liver tissue. For this purpose, 32 rats were treated for 28 days as follows: control group was injected intra-peritoneally with physiological saline; e-cigarette 0% treated group received an intra-peritoneal injection of e-liquid without nicotine diluted in physiological saline, e-cigarette-treated group received an intra-peritoneal injection of e-liquid containing 0.5 mg of nicotine/kg of body weight/day diluted in physiological saline and nicotine-treated group received an intra-peritoneal injection of 0.5 mg of nicotine/kg of body weight/day diluted in physiological saline. In e-liquid without nicotine-exposed group, activities of the liver biomarkers aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase increase. Interestingly, oxidative stress indicators showed decreased total protein content, associated with a reduction in the antioxidant enzymes activities superoxide dismutase, catalase and glutathione-S-transferase, and an elevation in malondialdehyde content, highlighting the promotion of lipid peroxidation and oxidative stress. Histological studies identified inflammatory cells infiltration and cell death. Thus, e-liquid seems to promote oxidative tissue injuries, which in turn lead to the observed histopathological finding. In comparison, nicotine alone induced less oxidative stress and less histopathological disorders, whereas e-liquid with nicotine gave rise to more histopathological injuries. Thereby, e-liquid, per se, is able to induce hepatotoxicity and supplementation with nicotine worsens this state.
ISSN:1537-6516
1537-6524
DOI:10.3109/15376516.2016.1160963