Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy

Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic,...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2016-07, Vol.100 (14), p.6231-6238
Main Authors: Xi, Tianqi, Kim, Dae Geun, Roh, Seong Woon, Choi, Jong-Soon, Choi, Yoon-E
Format: Article
Language:English
Subjects:
Additives
Algae
Analysis
Biomass
Biomedical and Life Sciences
Biosynthesis
Biotechnological Products and Process Engineering
Biotechnology
Carbon
Carotenoids
Cell division
Culture Media - chemistry
Cysts
Environmental conditions
Functional foods & nutraceuticals
Haematococcus pluvialis
Health aspects
Industrial Microbiology - methods
Life Sciences
Light
Light emitting diodes
Light sources
Microalgae
Microalgae - metabolism
Microbial Genetics and Genomics
Microbiology
Pigmentation
Productivity
Science
Studies
Volvocida - metabolism
Wavelengths
Xanthophylls - biosynthesis
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Summary:Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic, and pharmaceutical. However, producing astaxanthin from H. pluvialis necessitates separation with distinctive culture conditions, dividing between the microalgae growth and the astaxanthin production stages. Light-emitting diodes (LEDs) have emerged as a replacement for traditional light sources, and LED applications are now rapidly expanding to multiple areas in fields such as biotechnology. However, further detail application into microalgae biotechnology remains limited. In this study, we have attempted to establish new protocols based on the specific wavelength of LEDs for the cultivation and production of astaxanthin using H. pluvialis. Specifically, we applied red LEDs for microalgae cell growth and then switched to blue LEDs to induce astaxanthin biosynthesis. The result showed that astaxanthin productions based on a wavelength shift from red to blue were significantly increased, compared to those with continuous illumination using red LEDs. Furthermore, additional increase of astaxanthin production was achieved with simultaneous application of exogenous carbon with blue LED illumination. Our approach based on the proper manipulation of LED wavelengths upon H. pluvialis cell stages will enable the improvement of biomass and enhance astaxanthin production using H. pluvialis .
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-016-7301-6