Fractional laser-assisted drug delivery: Laser channel depth influences biodistribution and skin deposition of methotrexate

Background and Objective Ablative fractional laser (AFXL) facilitates delivery of topical methotrexate (MTX). This study investigates impact of laser‐channel depth on topical MTX‐delivery. Materials and Methods MTX (1% [w/v]) diffused for 21 hours through AFXL‐exposed porcine skin in in vitro Franz...

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Bibliographic Details
Published in:Lasers in surgery and medicine 2016-07, Vol.48 (5), p.519-529
Main Authors: Taudorf, E.H., Lerche, C.M., Erlendsson, A.M., Philipsen, P.A., Hansen, S.H., Janfelt, C., Paasch, U., Anderson, R.R., Haedersdal, M.
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Animals
Chromatography, High Pressure Liquid
Dermatologic Agents - administration & dosage
Dermatologic Agents - pharmacokinetics
Drug Delivery Systems - methods
Er:Yag laser
Female
fluorescence microscopy
Franz skin permeability Cells
high performance liquid chromatography
laser-assisted drug delivery
Lasers
Lasers, Solid-State
Methotrexate - administration & dosage
Methotrexate - pharmacokinetics
Microscopy, Fluorescence
Permeability
Skin - metabolism
Skin Absorption
Swine
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Summary:Background and Objective Ablative fractional laser (AFXL) facilitates delivery of topical methotrexate (MTX). This study investigates impact of laser‐channel depth on topical MTX‐delivery. Materials and Methods MTX (1% [w/v]) diffused for 21 hours through AFXL‐exposed porcine skin in in vitro Franz Cells (n = 120). A 2,940 nm AFXL generated microscopic ablation zones (MAZs) into epidermis (11 mJ/channel, MAZ‐E), superficial‐dermis (26 mJ/channel, MAZ‐DS), and mid‐dermis (256 mJ/channel, MAZ‐DM). High performance liquid chromatography (HPLC) was used to quantify MTX deposition in full‐thickness skin, biodistribution profiles at specific skin levels, and transdermal permeation. Fluorescence microscopy was used to visualize UVC‐activated MTX‐fluorescence (254 nm) and semi‐quantify MTX distribution in skin. Results AFXL increased topical MTX‐delivery (P 
ISSN:0196-8092
1096-9101
DOI:10.1002/lsm.22484