Comparative analysis of bioactive N-alkylamides produced by tissue culture raised versus field plantlets of Spilanthes ciliata using LC-Q-TOF (HRMS)

•High shoot regeneration (81%) for Spilanthes ciliata in 6 weeks of culture period was achieved using leaf discs as explants.•Maximum number of compounds eluted out in 1:2 (Plant:Solvent) ratio of extraction with ethanol.•Comparative analysis of 14 distinct alkylamides in tissue culture verses field...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2016-04, Vol.1017-1018, p.195-203
Main Authors: Bhat, Zubair Shanib, Jaladi, Neeharika, Khajuria, Ravi Kant, Shah, Zeeshan Hamid, Arumugam, Neelakantan
Format: Article
Language:English
Subjects:
Alkylamides
Amides - metabolism
Asteraceae - metabolism
Biocompatibility
Chromatography, High Pressure Liquid
Contamination
Culture
Disks
Elution
Ethanol extract
Extraction
LC–MS
Mass Spectrometry - methods
Mouth
Plants (organisms)
Spilanthes ciliata
Tissue culture
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Summary:•High shoot regeneration (81%) for Spilanthes ciliata in 6 weeks of culture period was achieved using leaf discs as explants.•Maximum number of compounds eluted out in 1:2 (Plant:Solvent) ratio of extraction with ethanol.•Comparative analysis of 14 distinct alkylamides in tissue culture verses field grown plants, tentatively identified by LC–HRMS. Spilanthes ciliata (S. ciliata) is a perennial herb of global importance owing to its luscious source of bioactive fatty acid derived amides known as N-alkylamides. It finds application in skin creams, mouth gels and toothpastes. Despite multifaceted applications, a major limitation associated for its commercial application is the scarcity of contamination free plant source, fluctuations in active metabolites due to variation in extraction procedures, and lack of rapid qualitative method for alkylamide profiling. In the current work, attempts were made to 1) optimize conditions for mass propagation of contamination free plants of S. ciliata by tissue culture using leaf discs as explants, 2) establish an optimum extraction ratio of plant/solvent (w/v) for maximum elution of alkylamides and 3) develop a rapid method for qualitative estimation of alkylamide from in vitro raised plants in comparison with that of the field grown counterpart by using LC-Q-TOF (HRMS). To the best of our knowledge, this is the first qualitative report on alkylamide profile of micropropagated whole plant of Spilanthes. The correlation pattern reported in this study may form the basis for using tissue culture raised plantlets of S. ciliata as potential source of bioactive alkylamides on industrial scale.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2016.02.023