Global Gene Deletion Analysis Exploring Yeast Filamentous Growth

The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2012-09, Vol.337 (6100), p.1353-1356
Main Authors: Ryan, Owen, Shapiro, Rebecca S., Kurat, Christoph F., Mayhew, David, Baryshnikova, Anastasia, Chin, Brian, Lin, Zhen-Yuan, Cox, Michael J., Vizeacoumar, Frederick, Cheung, Doris, Bahr, Sondra, Tsui, Kyle, Tebbji, Faiza, Sellam, Adnane, Istel, Fabian, Schwarzmüller, Tobias, Reynolds, Todd B., Kuchler, Karl, Gifford, David K., Whiteway, Malcolm, Giaever, Guri, Nislow, Corey, Costanzo, Michael, Gingras, Anne-Claude, Mitra, Robi David, Andrews, Brenda, Fink, Gerald R., Cowan, Leah E., Boone, Charles
Format: Article
Language:English
Subjects:
Alleles
Biofilms - growth & development
Biological and medical sciences
Biomedical research
Candida
Candida albicans
Candida albicans - cytology
Candida albicans - genetics
Candida albicans - growth & development
Cell growth
Deletion
Diploidy
DNA Mutational Analysis
Forages
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene Expression Regulation, Fungal
Genes
Genomes
Genotype & phenotype
Haploidy
Hyphae - genetics
Hyphae - growth & development
Medical research
Microbiology
Morphology
Morphology, structure, chemical composition
Mycology
Nuclear Proteins - genetics
Nutrients
Pathogens
Phenotypes
Regulator genes
Regulators
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins - genetics
Trans-Activators - genetics
Transcription factors
Transcription Factors - genetics
Transcription, Genetic
Yeast
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Description
Summary:The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, Σ1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.1224339