Functional double-shelled silicon nanocrystals for two-photon fluorescence cell imaging: spectral evolution and tuning

Functional near-IR (NIR) emitting nanoparticles (NPs) adapted for two-photon excitation fluorescence cell imaging were obtained starting from octadecyl-terminated silicon nanocrystals (ncSi-OD) of narrow photoluminescence (PL) spectra having no long emission tails, continuously tunable over the 700-...

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Bibliographic Details
Published in:Nanoscale 2016-01, Vol.8 (16), p.9009-9019
Main Authors: Chandra, Sourov, Ghosh, Batu, Beaune, Grégory, Nagarajan, Usharani, Yasui, Takao, Nakamura, Jin, Tsuruoka, Tohru, Baba, Yoshinobu, Shirahata, Naoto, Winnik, Françoise M
Format: Article
Language:English
Subjects:
Animals
Fluorescence
HEK293 Cells
Humans
Imaging
Mice
Microscopy, Fluorescence, Multiphoton - methods
Nanocrystals
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Nanostructure
NIH 3T3 Cells
Optical Phenomena
Particle Size
Pluronic F127
Quantum Dots - chemistry
Quantum Dots - ultrastructure
Silicon
Silicon - chemistry
Spectroscopy, Fourier Transform Infrared
Spectroscopy, Near-Infrared
Tuning
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Summary:Functional near-IR (NIR) emitting nanoparticles (NPs) adapted for two-photon excitation fluorescence cell imaging were obtained starting from octadecyl-terminated silicon nanocrystals (ncSi-OD) of narrow photoluminescence (PL) spectra having no long emission tails, continuously tunable over the 700-1000 nm window, PL quantum yields exceeding 30%, and PL lifetimes of 300 μs or longer. These NPs, consisting of a Pluronic F127 shell and a core made up of assembled ncSi-OD kept apart by an octadecyl (OD) layer, were readily internalized into the cytosol, but not the nucleus, of NIH3T3 cells and were non-toxic. Asymmetrical field-flow fractionation (AF4) analysis was carried out to determine the size of the NPs in water. HiLyte Fluor 750 amine was linked via an amide link to NPs prepared with Pluronic-F127-COOH, as a first demonstration of functional NIR-emitting water dispersible ncSi-based nanoparticles.
ISSN:2040-3364
2040-3372
DOI:10.1039/c6nr01437b