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Selective nitrate detection by an enzymatic sensor based on an extended-gate type organic field-effect transistor
First selective nitrate biosensor device based on an extended-gate type organic field-effect transistor (OFET) is reported. The fabricated sensor device consists of the extended-gate electrode functionalized by a nitrate reductase with a mediator (=a bipyridinium derivative) and an OFET-based transd...
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Published in: | Biosensors & bioelectronics 2016-07, Vol.81, p.87-91 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | First selective nitrate biosensor device based on an extended-gate type organic field-effect transistor (OFET) is reported. The fabricated sensor device consists of the extended-gate electrode functionalized by a nitrate reductase with a mediator (=a bipyridinium derivative) and an OFET-based transducer. The mechanism of the nitrate detection can be explained by an electron-relay on the extended-gate electrode, resulting in changes of the electric properties of the OFET. The detection limit of nitrate in water is estimated to be 45 ppb, which suggests that the sensitivity of our fabricated sensor is comparable to those of some conventional detection methods. As a practical application of the OFET sensor, the nitrate detection in diluted human saliva has been successfully demonstrated; the results agreed well with those by conventional colorimetric measurement. The advantages of OFETs are printability, mechanical flexibility, stretchability and disposability, meaning that the fabricated OFET could open up a new approach for low-cost electronic devices toward on-site detection of nitrate in aqueous media.
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•An enzymatic OFET biosensor detected nitrate selectively in aqueous media.•The limit of detection for lactate was estimated to be 45 ppb.•The designed OFET can pave the way for the detection of nitrate in human saliva. |
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ISSN: | 0956-5663 1873-4235 |
DOI: | 10.1016/j.bios.2016.02.036 |