Selective nitrate detection by an enzymatic sensor based on an extended-gate type organic field-effect transistor

First selective nitrate biosensor device based on an extended-gate type organic field-effect transistor (OFET) is reported. The fabricated sensor device consists of the extended-gate electrode functionalized by a nitrate reductase with a mediator (=a bipyridinium derivative) and an OFET-based transd...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2016-07, Vol.81, p.87-91
Main Authors: Minami, Tsuyoshi, Sasaki, Yui, Minamiki, Tsukuru, Wakida, Shin-ichi, Kurita, Ryoji, Niwa, Osamu, Tokito, Shizuo
Format: Article
Language:English
Subjects:
Biosensing Techniques - instrumentation
Biosensors
Devices
Electrodes
Enzymatic sensor
Enzymes, Immobilized - chemistry
Equipment Design
Field effect transistors
Humans
Limit of Detection
Nitrate
Nitrate reductase
Nitrate Reductase - chemistry
Nitrates
Nitrates - analysis
Organic electronics
Organic field-effect transistor
Saliva - chemistry
Semiconductor devices
Sensors
Transducers
Transistors, Electronic
Water - analysis
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Summary:First selective nitrate biosensor device based on an extended-gate type organic field-effect transistor (OFET) is reported. The fabricated sensor device consists of the extended-gate electrode functionalized by a nitrate reductase with a mediator (=a bipyridinium derivative) and an OFET-based transducer. The mechanism of the nitrate detection can be explained by an electron-relay on the extended-gate electrode, resulting in changes of the electric properties of the OFET. The detection limit of nitrate in water is estimated to be 45 ppb, which suggests that the sensitivity of our fabricated sensor is comparable to those of some conventional detection methods. As a practical application of the OFET sensor, the nitrate detection in diluted human saliva has been successfully demonstrated; the results agreed well with those by conventional colorimetric measurement. The advantages of OFETs are printability, mechanical flexibility, stretchability and disposability, meaning that the fabricated OFET could open up a new approach for low-cost electronic devices toward on-site detection of nitrate in aqueous media. [Display omitted] •An enzymatic OFET biosensor detected nitrate selectively in aqueous media.•The limit of detection for lactate was estimated to be 45 ppb.•The designed OFET can pave the way for the detection of nitrate in human saliva.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2016.02.036