Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases

Fresh fruit is highly perishable during postharvest life, mainly due to fungal growth. Thus, fungal control is an important goal for the fruit industry. In this work, a selection of antagonistic yeasts isolated from fig and breba crops were screened in vitro. The isolated yeasts were challenged with...

Full description

Saved in:
Bibliographic Details
Published in:Food microbiology 2016-08, Vol.57, p.45-53
Main Authors: Ruiz-Moyano, S., Martín, A., Villalobos, M.C., Calle, A., Serradilla, M.J., Córdoba, M.G., Hernández, A.
Format: Article
Language:English
Subjects:
Antagonistic yeast
Antibiosis
Botrytis - growth & development
Botrytis cinerea
Candida - growth & development
Citrus
Cladosporium cladosporioides
Crops
Ficus - microbiology
Ficus carica
Fruit - microbiology
Fruits
Fungi
Hanseniaspora
Hanseniaspora opuntiae
Malus - microbiology
Metschnikowia pulcherrima
Molds
Monilia
Monilia laxa
Moulds
PDA
Penicillium
Penicillium - growth & development
Penicillium expansum
Plant Diseases - microbiology
Plant Diseases - prevention & control
Plant Nectar
Postharvest disease
Yeast
Yeasts - genetics
Yeasts - isolation & purification
Yeasts - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fresh fruit is highly perishable during postharvest life, mainly due to fungal growth. Thus, fungal control is an important goal for the fruit industry. In this work, a selection of antagonistic yeasts isolated from fig and breba crops were screened in vitro. The isolated yeasts were challenged with three moulds isolated from decayed figs and breba crops, identified as Penicillium expansum M639 and Cladosporium cladosporioides M310 and M624, and pathogenic moulds Botrytis cinerea CECT20518 and Monilia laxa CA1 from culture collections. Two yeast isolates, Hanseniaspora opuntiae L479 and Metschnikowia pulcherrima L672, were selected for their ability to inhibit the growth of aforementioned moulds. These yeasts reduced the radial growth of moulds on PDA by between 45.23% and 66.09%. Antagonistic activity was associated with the interaction of live yeast cells with moulds. M. pulcherrima L672 apparently parasitised C. cladosporioides isolates. In addition, challenges were assayed using wounded apples and nectarines, with significant reductions in percent infection and lesion size for all moulds tested. To our knowledge, this is the first report identifying H. opuntiae as an antagonist against different pathogenic moulds. •The 34.5% of isolated yeasts showed antagonistic activity against pathogenic moulds.•H. opuntiae L479 and M. pulcherrima L672 were selected based on in vitro tests.•Selected yeasts were effective at controlling mould growth on apples and nectarines.•First report of the potential use of H. opuntiae for the biocontrol of different postharvest pathogens.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2016.01.003