HILIC-MS for metabolomics: An attractive and complementary approach to RPLC-MS

Hydrophilic interaction chromatography (HILIC) is an emerging separation mode of liquid chromatography (LC). Using highly hydrophilic stationary phases capable of retaining polar/ionic metabolites, and accompany with high organic content mobile phase that offer readily compatibility with mass spectr...

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Bibliographic Details
Published in:Mass spectrometry reviews 2016-09, Vol.35 (5), p.574-600
Main Authors: Tang, Dao-Quan, Zou, Ll, Yin, Xiao-Xing, Ong, Choon Nam
Format: Article
Language:English
Subjects:
Animals
Chromatography, Liquid
Chromatography, Reverse-Phase
Humans
hydrophilic interaction chromatography
Hydrophobic and Hydrophilic Interactions
mass spectrometry
metabolic-profiling
Metabolomics
polar compounds
reverse-phase chromatography
Tandem Mass Spectrometry
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Summary:Hydrophilic interaction chromatography (HILIC) is an emerging separation mode of liquid chromatography (LC). Using highly hydrophilic stationary phases capable of retaining polar/ionic metabolites, and accompany with high organic content mobile phase that offer readily compatibility with mass spectrometry (MS) has made HILIC an attractive complementary tool to the widely used reverse‐phase (RP) chromatographic separations in metabolomic studies. The combination of HILIC and RPLC coupled with an MS detector expands the number of detected analytes and provides more comprehensive metabolite coverage than use of only RP chromatography. This review describes the recent applications of HILIC‐MS/MS in metabolomic studies, ranging from amino acids, lipids, nucleotides, organic acids, pharmaceuticals, and metabolites of specific nature. The biological systems investigated include microbials, cultured cell line, plants, herbal medicine, urine, and serum as well as tissues from animals and humans. Owing to its unique capability to measure more‐polar biomolecules, the HILIC separation technique would no doubt enhance the comprehensiveness of metabolite detection, and add significant value for metabolomic investigations. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:574–600, 2016
ISSN:0277-7037
1098-2787
DOI:10.1002/mas.21445