TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis

Propolis is a “natural” remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and...

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Bibliographic Details
Published in:Journal of chromatographic science 2016-08, Vol.54 (7), p.1077-1083
Main Authors: Milojković Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena, Vovk, Irena, Lušić, Dražen, Tešić, Živoslav
Format: Article
Language:English
Subjects:
Animals
Bees - physiology
Chromatography, Thin Layer - standards
Croatia
Flavonoids - isolation & purification
Humans
Image Processing, Computer-Assisted - statistics & numerical data
Phenols - isolation & purification
Populus
Principal Component Analysis
Propolis - chemistry
Resins, Plant - chemistry
Serbia
Slovenia
Waxes - chemistry
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Summary:Propolis is a “natural” remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and chemometric approach for phenolic profiling of Serbian, Croatian and Slovenian propolis test solutions. Modern thin-layer chromatography equipment in combination with software for image processing was applied for fingerprinting and data acquisition, whereas the principal component analysis was used as pattern recognition method. Characterization of phenolic profile was performed along with the determination of the botanical and geographical origin of propolis. High-performance thin-layer chromatograms reveal that Central and Southeastern European propolis samples are rich in flavonoids. In addition, phenolic compounds proved to be suitable markers for the determination of European propolis authenticity.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmw024