Tertiary Dentin Formation after Indirect Pulp Capping Using Protein CPNE7

If there is a partial loss of dentin, the exposed dentinal surface should be protected by an indirect pulp capping (IPC) procedure to preserve pulp vitality and prevent symptoms of dentin hypersensitivity. In our previous study, copine7 (CPNE7) induced odontoblast differentiation in vitro and promot...

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Bibliographic Details
Published in:Journal of dental research 2016-07, Vol.95 (8), p.906-912
Main Authors: Choung, H.W., Lee, D.S., Lee, Ji-Hyun, Shon, W.J., Lee, Jong-Ho, Ku, Y., Park, J.C.
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Calcium
Canine teeth
Cell adhesion & migration
Cell Movement - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Dental pulp
Dental Pulp Capping - methods
Dentin
Dentin - drug effects
Dentin - growth & development
Dentistry
Dogs
Experiments
Fluorides
Humans
Hypersensitivity
Membrane Proteins - therapeutic use
Mineralization
Odontoblasts
Odontoblasts - drug effects
Odontoblasts - physiology
Proteins
Real-Time Polymerase Chain Reaction
Scanning electron microscopy
Teeth
Tubules
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Summary:If there is a partial loss of dentin, the exposed dentinal surface should be protected by an indirect pulp capping (IPC) procedure to preserve pulp vitality and prevent symptoms of dentin hypersensitivity. In our previous study, copine7 (CPNE7) induced odontoblast differentiation in vitro and promoted dentin formation in vivo. The aim of this study was to investigate the possibility of IPC therapy using the CPNE7 protein at the exposed dentinal surface and the resulting effects on tertiary dentin formation in a beagle model. CPNE7 promoted mineralization of odontoblasts and had high calcium ion-binding capacity. The in vivo IPC model with canine teeth showed that regeneration of physiologic reactionary dentin with dentinal tubule structures was clearly observed beneath the remaining dentin in the CPNE7 group, whereas irregular features of reparative dentin were generated in the mineral trioxide aggregate (MTA) group. The CPNE7+MTA group also showed typical reactionary dentin without reparative dentin, showing synergistic effects of CPNE7 with MTA. A scanning electron microscopy analysis showed that dentinal tubules beneath the original dentin were occluded by the deposition of peritubular dentin in the CPNE7 and CPNE7+MTA groups, whereas those in the control group were opened. Therefore, CPNE7 may be able to serve as a novel IPC material and improve symptoms of dentin hypersensitivity.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034516639919