Changes in ABCB1 mRNA Expression in Peripheral Blood Cells before and after Renal Transplantation

Cyclosporine (CSA), which is one of the substrates of ATP binding cassette subfamily B member 1 (ABCB1), is widely used as an immunosuppressant in patients undergoing transplantation. The expression level of P-glycoprotein on lymphocytes that is encoded by ABCB1 gene is considered to be one of the m...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2016/07/01, Vol.39(7), pp.1085-1090
Main Authors: Kushihara, Hideyuki, Kuzuya, Takafumi, Miwa, Yuko, Iwasaki, Kenta, Watarai, Yoshihiko, Uchida, Kazuharu, Yamada, Kiyofumi, Kobayashi, Takaaki
Format: Article
Language:eng ; jpn
Subjects:
Adult
Aged
ATP binding cassette subfamily B member 1 (ABCB1)
ATP Binding Cassette Transporter, Sub-Family B - genetics
Cyclosporine - blood
Cyclosporine - pharmacokinetics
Cyclosporine - pharmacology
Cyclosporine - therapeutic use
cyclosporine A
Cytomegalovirus Infections
Female
Graft Rejection
Humans
Immunosuppressive Agents - blood
Immunosuppressive Agents - pharmacokinetics
Immunosuppressive Agents - pharmacology
Immunosuppressive Agents - therapeutic use
Kidney Transplantation
Leukocytes, Mononuclear - metabolism
Male
Middle Aged
mRNA
Recurrence
renal transplantation
RNA, Messenger - blood
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Summary:Cyclosporine (CSA), which is one of the substrates of ATP binding cassette subfamily B member 1 (ABCB1), is widely used as an immunosuppressant in patients undergoing transplantation. The expression level of P-glycoprotein on lymphocytes that is encoded by ABCB1 gene is considered to be one of the major causes of differences in intracellular CSA concentration. The clinical relevance of ABCB1 mRNA expression in peripheral blood was analyzed. We examined (i) the relationship between ABCB1 mRNA and the intracellular concentration of CSA in vitro, (ii) the change in long-term ABCB1 mRNA expression levels, and (iii) its association with acute rejection (AR) or cytomegalovirus (CMV) reactivation in living-donor renal transplantation. A significantly negative correlation between ABCB1 mRNA expression and intracellular CSA concentration in vitro was obtained (p
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b15-00690