Identification of an octanucleotide motif sequence essential for viral protein, DNA, and progeny virus biosynthesis at the origin of DNA replication of porcine circovirus type 2

A plasmid-based transfection system capable of generating infectious porcine circovirus type 2 (PCV2) was established. This system was then used in mutagenesis studies to investigate the involvement of a “conserved” nonanucleotide (which constitutes a portion of the loop sequence) located at the ori...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2004-06, Vol.324 (1), p.28-36
Main Author: Cheung, Andrew K
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Circovirus - genetics
DNA Replication
DNA, Viral - biosynthesis
Genotype
Molecular Sequence Data
nucleotide sequences
Oligonucleotides - chemistry
Origin of DNA replication
Porcine circovirus
Porcine circovirus 2
Replication Origin
Research Design
Swine
transfection
vertebrate viruses
Viral Proteins - biosynthesis
Virus Replication
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Summary:A plasmid-based transfection system capable of generating infectious porcine circovirus type 2 (PCV2) was established. This system was then used in mutagenesis studies to investigate the involvement of a “conserved” nonanucleotide (which constitutes a portion of the loop sequence) located at the origin of DNA replication of PCV2 with respect to viral protein synthesis, DNA self-replication, and progeny virus production. The results demonstrated that an octanucleotide (AGTATTAC) embedded in the loop sequence is essential for virus replication. This octanucleotide can be further condensed to an essential core element (ECE) represented by AxTAxTAC. The positions specified by the indicated nucleotides are critical for viral DNA replication and stable infectious virus production, and they cannot be substituted by other bases, while the positions indicated by x can accept variable bases and yield stable progeny viruses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2004.03.037