Uptake and Toxicity Studies of Poly-Acrylic Acid Functionalized Silicon Nanoparticles in Cultured Mammalian Cells

Poly‐acrylic acid (PAAc) terminated silicon nanoparticles (SiNPs) have been synthesized and employed as a synchronous fluorescent signal indicator in a series of cultured mammalian cells: HHL5, HepG2 and 3T3‐L1. Their biological effects on cell growth and proliferation in both human and mouse cell l...

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Bibliographic Details
Published in:Advanced healthcare materials 2012-03, Vol.1 (2), p.189-198
Main Authors: Wang, Qi, Bao, Yongping, Zhang, Xiaohong, Coxon, Paul R., Jayasooriya, Upali A., Chao, Yimin
Format: Article
Language:English
Subjects:
3T3 Cells
Acrylic Resins - chemistry
Animals
Cell growth
Cell Proliferation - drug effects
cell proliferation and viability
Cell Survival - drug effects
cytotoxicity
Diffusion
Encapsulation
Exposure
fluorescence
Hep G2 Cells
Humans
In vitro testing
Mammals
Materials Testing
Mice
Micelles
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - toxicity
Silicon
Silicon - chemistry
Silicon - toxicity
Uptakes
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Summary:Poly‐acrylic acid (PAAc) terminated silicon nanoparticles (SiNPs) have been synthesized and employed as a synchronous fluorescent signal indicator in a series of cultured mammalian cells: HHL5, HepG2 and 3T3‐L1. Their biological effects on cell growth and proliferation in both human and mouse cell lines have been studied. There was no evidence of in vitro cytotoxity in the cells exposed to PAAc terminated SiNPS when assessed by cell morphology, cell proliferation and viability, and DNA damage assays. The uptake of the nanocrystals by both HepG2 and 3T3‐L1 cells was investigated by confocal microscopy and flow cytometry, which showed a clear time‐dependence at higher concentrations. Reconstructed 3‐D confocal microscope images exhibited that the PAAc‐SiNPs were evenly distributed throughout the cytosol rather than attached to outer membrane. This study provides fundamental evidence for the safe application and further modification of silicon nanoparticles, which could broaden their application as cell markers in living systems and in micelle encapsulated drug delivery systems. Poly‐acrylic acid grafted silicon nanoparticles are bio‐compatible and can be suspended in water. No evidence of in vitro cytotoxicity is demonstrated upon exposure of cells to the nanoparticles. When incubated into mammalian cells, the nanoparticles display intense luminescence, and the cellular uptake of the nanoparticles shows a clear time dependence at higher concentrations. This study provides fundamental evidence for safe application of silicon nanoparticles, which could broaden their potential uses as cell markers in living systems and in micelle encapsulated drug‐delivery systems.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201100010