Highly reproducible skeletal deformities induced by administration of β-aminopropionitrile to developing chick embryos

The formation of cross-linkages between and within collagen is catalyzed by lysyl oxidase, which can be inhibited by β-aminopropionitrile (BAPN), a lathyrogen from sweet pea (Lathyrus odoratus) seeds. The quality and integrity of the collagenous template of skeletal elements depend on an appropriate...

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Bibliographic Details
Published in:Journal of Oral Science 2016, Vol.58(2), pp.255-263
Main Authors: Kubota, Sakura, Yuguchi, Maki, Yamazaki, Yosuke, Kanazawa, Hirofumi, Isokawa, Keitaro
Format: Article
Language:English
Subjects:
Aminopropionitrile - toxicity
Animals
Bone and Bones - drug effects
bone development
Chick Embryo
Dentistry
Dose-Response Relationship, Drug
lathyrogen
Reproducibility of Results
skeletal deformity
β-aminopropionitrile
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Summary:The formation of cross-linkages between and within collagen is catalyzed by lysyl oxidase, which can be inhibited by β-aminopropionitrile (BAPN), a lathyrogen from sweet pea (Lathyrus odoratus) seeds. The quality and integrity of the collagenous template of skeletal elements depend on an appropriate concentration of collagen cross-links. In this study, chick embryos treated in ovo with BAPN on embryonic days (ED) 4-9 were found to develop multiple skeletal deformities. The most readily discernible and highly reproducible deformity was evident in the tibiotarsus, on which we focused to explore the chronology of the malformation process. Several lines of observation indicated that the bending deformity observable at ED10 in the tibiotarsus was inducible by BAPN administered on ED4-8; in other words, administration of BAPN on ED8 was sufficient to induce the deformity by ED10, whereas administration on ED9 was ineffective. Ultrastructurally, osteoclasts appeared to show enhanced activity in the medullary surface of the bone collar after BAPN administration. In addition, bone hyperplasia associated with the bending deformity was suggested to be correlated with higher osteoblast activity on the concave (or flexor) side of the tibiotarsal skeleton. These findings indicate that the bending deformity due to reduced mechanical integrity of the collagenous template is also associated with aberrant bone remodeling. (J Oral Sci 58, 255-263, 2016)
ISSN:1343-4934
1880-4926
DOI:10.2334/josnusd.15-0723