Kit ligand promotes the transition from primordial to primary follicles after in vitro culture of ovine ovarian tissue

This study evaluated the effects of kit ligand (KL) on the morphology and development of ovine preantral follicles (fresh control) and after 7 days of in vitro culture in α-Minimal Essential Medium (α-MEM; control medium) or the presence of KL (1, 10, 50, 100 or 200 ng/ml). There was an increase in...

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Bibliographic Details
Published in:Zygote (Cambridge) 2016-08, Vol.24 (4), p.578-582
Main Authors: Cavalcante, A.Y.P., Gouveia, B.B., Barberino, R.S., Lins, T.L.B.G., Santos, L.P., Gonçalves, R.J.S., Celestino, J.J.H., Matos, M.H.T.
Format: Article
Language:English
Subjects:
Animals
Culture Media - chemistry
Culture Media - pharmacology
Dose-Response Relationship, Drug
Female
Organic Chemicals - chemistry
Organic Chemicals - pharmacology
Ovarian Follicle - drug effects
Ovarian Follicle - physiology
Ovary - drug effects
Ovary - physiology
Sheep
Short Communication
Stem Cell Factor - pharmacology
Time Factors
Tissue Culture Techniques
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Summary:This study evaluated the effects of kit ligand (KL) on the morphology and development of ovine preantral follicles (fresh control) and after 7 days of in vitro culture in α-Minimal Essential Medium (α-MEM; control medium) or the presence of KL (1, 10, 50, 100 or 200 ng/ml). There was an increase in the percentage of primary follicles at the concentration of 100 ng/ml KL, compared with the fresh control, control medium (α-MEM) and the other KL concentrations. Follicle diameter was significantly higher than the control medium only at concentrations of 50 and 100 ng/ml KL. In conclusion, 100 ng/ml KL promoted the transition from primordial to primary follicles (follicular activation) after in vitro culture of ovine ovarian tissue.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199415000556