Gram-Positive Bacteria Are a Major Reservoir of Class 1 Antibiotic Resistance Integrons in Poultry Litter

Reversing the spread of antibiotic multiresistant bacteria is hampered by ignorance of the natural history of resistance genes, the mobile elements carrying them, and the bacterial hosts harboring them. Using traditional cultivation and cultivation-independent molecular techniques, we quantified ant...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2004-05, Vol.101 (18), p.7118-7122
Main Authors: Nandi, Sobhan, Maurer, John J., Hofacre, Charles, Summers, Anne O., Tiedje, James M.
Format: Article
Language:English
Subjects:
Agroecosystems
Animals
Antibiotic resistance
Antibiotics
Bacteria
Biological Sciences
Chickens - microbiology
Crops
Disease Reservoirs
Drug Resistance, Bacterial - genetics
Drug Resistance, Bacterial - physiology
Enterobacteriaceae
Flocks
Genetics
Genomes
Gram positive bacteria
Gram-Positive Bacteria - genetics
Gram-Positive Bacteria - metabolism
Integrases - genetics
Integrases - metabolism
Integrons
Microbiology
Poultry
Poultry litter
Staphylococcus
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Summary:Reversing the spread of antibiotic multiresistant bacteria is hampered by ignorance of the natural history of resistance genes, the mobile elements carrying them, and the bacterial hosts harboring them. Using traditional cultivation and cultivation-independent molecular techniques, we quantified antibiotic resistance genes and mobile elements called integrons in poultry house litter from commercial poultry farms. Unexpectedly, the major reservoir for Class 1 integrons in poultry litter is not their previously identified hosts, Gram-negative Enterobacteriaceae such as Escherichia coli. Rather, integrons and associated resistance genes abound in several genera of Gram-positive bacteria that constitute > 85% of the litter community compared with Enterobacteriaceae that comprise
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0306466101