Species clarification of Isaria isolates used as biocontrol agents against Diaphorina citri (Hemiptera: Liviidae) in Mexico

Entomopathogenic fungi belonging to the genus Isaria (Hypocreales: Cordycipitaceae) are promising candidates for microbial control of insect pests. Currently, the Mexican government is developing a biological control program based on extensive application of Isaria isolates against Diaphorina citri...

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Bibliographic Details
Published in:Fungal biology 2016-03, Vol.120 (3), p.414-423
Main Authors: Gallou, Adrien, Serna-Domínguez, María G., Berlanga-Padilla, Angélica M., Ayala-Zermeño, Miguel A., Mellín-Rosas, Marco A., Montesinos-Matías, Roberto, Arredondo-Bernal, Hugo C.
Format: Article
Language:English
Subjects:
Animals
Biological control
Citrus
Citrus - parasitology
Cluster Analysis
Diaphorina citri
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Entomopathogenic fungi
Genotype
Hemiptera
Hemiptera - microbiology
Hypocreales - classification
Hypocreales - cytology
Hypocreales - genetics
Hypocreales - isolation & purification
Isaria javanica
ISSR markers
Mexico
Microscopy
Molecular Sequence Data
Morphology
Peptide Elongation Factor 1 - genetics
Pest Control, Biological
Phenotype
Phylogeny
Plant Diseases - parasitology
Plant Diseases - prevention & control
Sequence Analysis, DNA
Tubulin - genetics
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Summary:Entomopathogenic fungi belonging to the genus Isaria (Hypocreales: Cordycipitaceae) are promising candidates for microbial control of insect pests. Currently, the Mexican government is developing a biological control program based on extensive application of Isaria isolates against Diaphorina citri (Hemiptera: Liviidae), a vector of citrus huanglongbing disease. Previous research identified three promising Isaria isolates (CHE-CNRCB 303, 305, and 307; tentatively identified as Isaria fumosorosea) from Mexico. The goal of this work was to obtain a complete morphological and molecular characterization of these isolates. Comparative analysis of morphology established that the isolates showed similar characteristics to Isaria javanica. Multi-gene analysis confirmed the morphological identification by including the three isolates within the I. javanica clade. Additionally, this work demonstrated the misidentifications of three other Isaria isolates (CHE-CNRCB 310 and 324: I. javanica, formerly I. fumosorosea; CHE-CNRCB 393: I. fumosorosea, formerly Isaria farinosa), underlying the need for a full and correct characterization of an isolate before developing a biological control program. Finally, the inter-simple sequence repeat (ISSR) genotyping method revealed that the CHE-CNRCB 303, 305, and 307 isolates belong to three different genotypes. This result indicates that ISSR markers could be used as a tool to monitor their presence in field conditions. •We characterized three Isaria isolates used in biological control program in Mexico.•Morphology established that isolates presented similar characteristics to Isaria javanica.•Multi-gene analysis confirmed the identification of the isolates as I. javanica.•ISSR genotyping revealed that the isolates belong to three different genotypes.•ISSR markers could be used as a powerful tool to monitor Isaria isolates in field.
ISSN:1878-6146
1878-6162
DOI:10.1016/j.funbio.2015.11.009