On-site hydrolytic enzymes production from fungal co-cultivation of Bermuda grass and corn cob

•On-site enzymes were produced from fungal fermentation of Bermuda grass and corn cob.•Fermented forage showed high cellulolytic, amylolytic and xylanolytic productivities.•Fermented forage showed potential in ruminal digestibility improvement of animal feed.•On-site enzymes production could represe...

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Bibliographic Details
Published in:Bioresource technology 2016-07, Vol.212, p.334-337
Main Authors: Amaro-Reyes, Aldo, Gracida, Jorge, Huizache-Peña, Nelson, Elizondo-García, Norberto, Salazar-Martínez, José, García Almendárez, Blanca E., Regalado, Carlos
Format: Article
Language:English
Subjects:
Animal Feed
Animals
Aspergillus niger
Aspergillus niger - enzymology
Aspergillus niger - growth & development
Carbohydrate Metabolism
Cellulose - metabolism
Co-culture
Coculture Techniques
Cynodon - microbiology
Digestion
Enzymes - metabolism
Fatty Acids, Volatile - metabolism
Fermentation
Hydrolysis
Hydrolytic enzymes
Hypocrea jecorina
Lignin - metabolism
On-site enzymes production
Polysaccharides - metabolism
Ruminantia
Ruminants
Trichoderma - enzymology
Trichoderma - growth & development
Trichoderma reesei
Zea mays - microbiology
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Summary:•On-site enzymes were produced from fungal fermentation of Bermuda grass and corn cob.•Fermented forage showed high cellulolytic, amylolytic and xylanolytic productivities.•Fermented forage showed potential in ruminal digestibility improvement of animal feed.•On-site enzymes production could represent economical availability of animal feed. Solid state fermentation (SSF) is used to produce industrial enzymes. The objective of this study was to use a co-culture of Aspergillus niger GS1 and Trichoderma reesei, grown on a mixture of Bermuda grass and corn cob to obtain fermented forage (FF) rich in hydrolytic enzymes, as a value added ingredient for animal feed. FPase, amylase and xylanase productivities (dry matter, DM) were 8.8, 181.4, and 42.1Ug−1h−1, respectively (1U=reducing sugars released min−1), after 12–16h of SSF with C/N=60. Cellulose, hemicellulose and lignin decreased 1.6-, 2.7- and 1.9-fold (DM), respectively. In vitro ruminal and true digestibility of DM was improved 2.4- and 1.4-fold. Ruminal digestion of FF reduced 1.32-fold the acetate:propionate ratio, which may reduce the environmental impact of ruminants feeding. On-site hydrolytic enzymes productivity using SSF without enzymes extraction could be of economic potential for digestibility improvement in animal feed.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.04.070