Heterotrophic nitrogen removal by a newly-isolated alkalitolerant microorganism, Serratia marcescens W5

•A new microbe, Serratia marcescens W5 was successfully isolated & further studied.•Serratia marcescens W5 had a maximum ammonium removal rate of 514.13mgL−1d−1.•W5 showed great ammonium removal abilities in a wide range of pH, Temp. & salinity.•Nitrite & nitrate were efficiently utilize...

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Bibliographic Details
Published in:Bioresource technology 2016-07, Vol.211, p.618-627
Main Authors: Wang, Teng, Dang, Qifeng, Liu, Chengsheng, Yan, Jingquan, Fan, Bing, Cha, Dongsu, Yin, Yanyan, Zhang, Yubei
Format: Article
Language:English
Subjects:
Aerobiosis
Alkalitolerant microorganism
Ammonium Compounds - metabolism
Denitrification
Heterotrophic nitrogen removal
Heterotrophic Processes
Nitrates - metabolism
Nitrites - metabolism
Nitrogen - isolation & purification
Nitrogen - metabolism
Nitrogen removal pathway
Serratia marcescens
Serratia marcescens - metabolism
Stress resistance
Waste Water - chemistry
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Summary:•A new microbe, Serratia marcescens W5 was successfully isolated & further studied.•Serratia marcescens W5 had a maximum ammonium removal rate of 514.13mgL−1d−1.•W5 showed great ammonium removal abilities in a wide range of pH, Temp. & salinity.•Nitrite & nitrate were efficiently utilized by W5 only in the presence of ammonium. A new microbe, Serratia marcescens W5 was successfully isolated. Its feasibility in purification of excessively nitrogen-containing wastewater was evaluated using inorganic nitrogen media. Single factor tests showed that W5 exhibited high ammonium removal rates (above 80%) under different culture conditions (pH 7–10, C/N ratios of 6–20, 15–35°C, 0–2.5% of salinity, respectively). Besides various organic carbon sources, W5 was able to utilize calcium carbonate with 28.05% of ammonium removed. Further experiments indicated that W5 was capable of resisting high-strength ammonium (1200mg/L) with the maximum removal rate of 514.13mgL−1d−1. The nitrogen removal pathway of W5 was also tested, showing that both nitrite and nitrate were efficiently removed only in the presence of ammonium, with hydroxylamine as intermediate, which was different from the conventional nitrogen removal pathway. All the results verified that W5 was a good candidate for the purification of excessively nitrogenous wastewater.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.03.142