Resistance-nodulation-division efflux pump acrAB is modulated by florfenicol and contributes to drug resistance in the fish pathogen Piscirickettsia salmonis

Piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids, with serious economic consequences for the Chilean aquaculture industry. Oxytetracycline and florfenicol are the most frequently used antibiotics against P. salmonis, but routine...

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Bibliographic Details
Published in:FEMS microbiology letters 2016-06, Vol.363 (11), p.fnw102
Main Authors: Sandoval, Rodrigo, Oliver, Cristian, Valdivia, Sharin, Valenzuela, Karla, Haro, Ronie E., Sánchez, Patricio, Olavarría, Víctor H., Valenzuela, Paulina, Avendaño-Herrera, Rubén, Romero, Alex, Cárcamo, Juan G., Figueroa, Jaime E., Yáñez, Alejandro J.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Aquaculture
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Chile
Drug Resistance, Multiple, Bacterial - genetics
Ethidium - metabolism
Fish
Fishes - microbiology
Genes, MDR
Genome, Bacterial
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Microbial Sensitivity Tests
Microbiology
Mortality
Pathogens
Piscirickettsia - drug effects
Piscirickettsia - genetics
Piscirickettsia - pathogenicity
Resistance to control
Thiamphenicol - analogs & derivatives
Thiamphenicol - pharmacology
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Summary:Piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids, with serious economic consequences for the Chilean aquaculture industry. Oxytetracycline and florfenicol are the most frequently used antibiotics against P. salmonis, but routine use could contribute to drug resistance. This study identified differentiated florfenicol susceptibilities in two P. salmonis strains, LF-89 and AUSTRAL-005. The less susceptible isolate, AUSTRAL-005, also showed a high ethidium bromide efflux rate, indicating a higher activity of general efflux pump genes than LF-89. The P. salmonis genome presented resistance nodulation division (RND) family members, a family containing typical multidrug resistance-related efflux pumps in Gram-negative bacteria. Additionally, efflux pump acrAB genes were overexpressed in AUSTRAL-005 following exposure to the tolerated maximal concentration of florfenicol, in contrast to LF-89. These results indicate that tolerated maximum concentrations of florfenicol can modulate RND gene expression and increase efflux pump activity. We propose that the acrAB efflux pump is essential for P. salmonis survival at critical florfenicol concentrations and for the generation of antibiotic-resistant bacterial strains. Florfenicol modulates the RND efflux pump acrAB expression and contributes to drug resistance in the fish pathogen Piscirickettsia salmonis.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnw102