Phylogenetic analysis of a newfound bat-borne hantavirus supports a laurasiatherian host association for ancestral mammalian hantaviruses

Until recently, hantaviruses (family Bunyaviridae) were believed to originate from rodent reservoirs. However, genetically distinct hantaviruses were lately found in shrews and moles, as well as in bats from Africa and Asia. Bats (order Chiroptera) are considered important reservoir hosts for emergi...

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Published in:Infection, genetics and evolution genetics and evolution, 2016-07, Vol.41, p.113-119
Main Authors: Witkowski, Peter T., Drexler, Jan F., Kallies, René, Ličková, Martina, Bokorová, Silvia, Mananga, Gael D., Szemes, Tomáš, Leroy, Eric M., Krüger, Detlev H., Drosten, Christian, Klempa, Boris
Format: Article
Language:English
Subjects:
Africa
Animals
Bat
Biological Evolution
Caves
Chiroptera - virology
Disease Reservoirs
Gabon - epidemiology
Hantavirus
Hantavirus - classification
Hantavirus - genetics
Hantavirus - isolation & purification
Hantavirus Infections - epidemiology
Hantavirus Infections - veterinary
Hantavirus Infections - virology
Kidney - virology
Laurasiatheria
Makokou virus
Mammals
Organ Specificity
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
Sequence Analysis, DNA
Spleen - virology
Viral Load
Zoonosis
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Summary:Until recently, hantaviruses (family Bunyaviridae) were believed to originate from rodent reservoirs. However, genetically distinct hantaviruses were lately found in shrews and moles, as well as in bats from Africa and Asia. Bats (order Chiroptera) are considered important reservoir hosts for emerging human pathogens. Here, we report on the identification of a novel hantavirus, provisionally named Makokou virus (MAKV), in Noack's Roundleaf Bat (Hipposideros ruber) in Gabon, Central Africa. Phylogenetic analysis of the genomic l-segment showed that MAKV was the most closely related to other bat-borne hantaviruses and shared a most recent common ancestor with the Asian hantaviruses Xuan Son and Laibin. Breakdown of the virus load in a bat animal showed that MAKV resembles rodent-borne hantaviruses in its organ distribution in that it predominantly occurred in the spleen and kidney; this provides a first insight into the infection pattern of bat-borne hantaviruses. Ancestral state reconstruction based on a tree of l gene sequences of all relevant hantavirus lineages was combined with phylogenetic fossil host hypothesis testing, leading to a statistically significant rejection of the mammalian superorder Euarchontoglires (including rodents) but not the superorder Laurasiatheria (including shrews, moles, and bats) as potential hosts of ancestral hantaviruses at most basal tree nodes. Our data supports the emerging concept of bats as previously overlooked hantavirus reservoir hosts. •Novel tentative Hantavirus species (Makokou virus) in Hipposideros bat from Gabon•MAKV phylogenetically groups with novel hantaviruses from shrews, moles, and bats•Virus load in H. ruber resembles organ distribution usually found in rodent hosts.•Laurasiatheria are the most probable host ancestor for the genus Hantavirus.
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2016.03.036