Observation of Large, Non-covalent Globin Subassemblies in the similar to 3600 kDa Hexagonal Bilayer Hemoglobins by Electrospray Ionization Time-of-Flight Mass Spectrometry

A non-covalent globin subassembly comprising 12 globin chains (204 to 214 kDa) was observed directly by electrospray ionization time-of-flight mass spectrometry in the native hexagonal bilayer hemoglobins from the oligochaetes Lumbricus terrestris and Tubifex tubifex, the polychaetes Tylorrhynchus h...

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Bibliographic Details
Published in:Journal of molecular biology 2001-06, Vol.309 (3), p.563-570
Main Authors: Green, B N, Gotoh, T, Suzuki, T, Zal, F, Lallier, F H, Toulmond, A, Vinogradov, S N
Format: Article
Language:English
Subjects:
Alvinella pompejana
Amphitrite ornata
Arenicola marina
globins
Haemopis grandis
Lumbricus terrestris
Macrobdella decora
Myxicola infundibulum
Nephelopsis oscura
Tubifex tubifex
Tylorrhynchus heterochaetus
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Summary:A non-covalent globin subassembly comprising 12 globin chains (204 to 214 kDa) was observed directly by electrospray ionization time-of-flight mass spectrometry in the native hexagonal bilayer hemoglobins from the oligochaetes Lumbricus terrestris and Tubifex tubifex, the polychaetes Tylorrhynchus heterochaetus, Arenicola marina, Amphitrite ornata and Alvinella pompejana, the leeches Macrobdella decora, Haemopis grandis and Nephelopsis oscura and the chlorocruorin from the polychaete Myxicola infundibulum, over the pH range 3.5-7.0. The Hb from the deep-sea polychaete Alvinella exhibited in addition, peaks at similar to 107 kDa and at similar to 285 kDa, which were assigned to subassemblies of six globin chains and of 12 globin chains with three non-globin linker chains, respectively. The experimental masses decreased slightly with increased de-clustering potential (60 to 160 V) and were generally 0.1 to 0.2 % higher than the calculated masses, due probably to complexation with cations and water molecules. Copyright 2001 Academic Press
ISSN:0022-2836
1089-8638
DOI:10.1006/jmbi.2001.4704