Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2)

The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG‐63 and Saos‐2) was investigated. In particular, the expression of two important integrins,...

Full description

Saved in:
Bibliographic Details
Published in:Bioelectromagnetics 2003-07, Vol.24 (5), p.327-338
Main Authors: Santini, Maria Teresa, Rainaldi, Gabriella, Ferrante, Antonella, Indovina, Pietro Luigi, Vecchia, Paolo, Donelli, Gianfranco
Format: Article
Language:English
Subjects:
apoptosis
Apoptosis - radiation effects
bone cells
cancer cells
cell adhesion molecules
Cell Adhesion Molecules - metabolism
cell cycle phases
Cell Division - radiation effects
Cell Line, Tumor - metabolism
Cell Line, Tumor - radiation effects
Cell Size - radiation effects
Dose-Response Relationship, Radiation
Electricity
Electromagnetic Fields
ELF
Gene Expression Regulation, Neoplastic - radiation effects
Humans
Hyaluronan Receptors - metabolism
Integrin alpha2beta1 - metabolism
Integrin alpha5beta1 - metabolism
Osteosarcoma - metabolism
Osteosarcoma - pathology
Radiation Dosage
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG‐63 and Saos‐2) was investigated. In particular, the expression of two important integrins, VLA‐2, the receptor for collagen, and VLA‐5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG‐63 and Saos‐2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG‐63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis. Bioelectromagnetics 24:327‐338, 2003. © 2003 Wiley‐Liss, Inc.
ISSN:0197-8462
1521-186X
DOI:10.1002/bem.10113