RpA, an extracellular protease similar to the metalloprotease of serralysin family, is required for pathogenicity of Ralstonia pickettii

Aim To investigate the biochemical and functional properties of an extracellular protease, RpA, in Ralstonia pickettii WP1 isolated from water supply systems. Methods and Results An extracellular protease was identified and characterized from R. pickettii WP1. A mutant strain WP1M2 was created from...

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Bibliographic Details
Published in:Journal of applied microbiology 2015-10, Vol.119 (4), p.1101-1111
Main Authors: Chen, C.‐M., Liu, J.‐J., Chou, C.‐W., Lai, C.‐H., Wu, L.‐T.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
culture filtrates
cytotoxic effect
Cytotoxicity
Enzyme Stability
Escherichia coli
Metalloendopeptidases - chemistry
Metalloendopeptidases - genetics
Metalloendopeptidases - metabolism
metalloprotease
Molecular Sequence Data
Pathogenesis
Proteases
Pseudomonas fluorescens
Ralstonia pickettii
Ralstonia pickettii - chemistry
Ralstonia pickettii - enzymology
Ralstonia pickettii - genetics
Ralstonia pickettii - pathogenicity
Ralstonia pickettii WP1
Sequence Alignment
Virulence
Water supply
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Summary:Aim To investigate the biochemical and functional properties of an extracellular protease, RpA, in Ralstonia pickettii WP1 isolated from water supply systems. Methods and Results An extracellular protease was identified and characterized from R. pickettii WP1. A mutant strain WP1M2 was created from strain WP1 by mini‐Tn5 transposition. The culture filtrates from WP1M2 had a lower cytotoxic effect than the parental WP1 on several mammalian cell lines. Cloning and sequence analysis revealed the Tn5 transposon inserted at a protease gene (rpA) which is 81% homologous to prtA and aprX genes of Pseudomonas fluorescens. The rpA gene encodes a 482‐residue protein showing sequence similarity to metalloproteases of the serralysin family. The RpA protein was expressed in Escherichia coli using a pET expression vector and purified as a 55 kDa molecular weight protein. Furthermore, the protease activity of RpA was inhibited by protease inhibitor and heat treatment. Conclusions The in vitro cytotoxic activity of R. pickettii culture filtrates was attributed to RpA protease. Significance and Impact of the Study An extracellular protease, RpA, was identified from R. pickettii WP1 isolated from water supply system. The RpA metalloproteases is required for the pathogenicity of R. pickettii to mammalian cell lines.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12903