Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs

AIMS: The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. METHODS AND RESULTS: Two replicates of approximately 650 pigs each were weaned and randomly assig...

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Bibliographic Details
Published in:Journal of applied microbiology 2015-06, Vol.118 (6), p.1489-1497
Main Authors: Wells, J.E, Berry, E.D, Kalchayanand, N, Rempel, L.A, Kim, M, Oliver, W.T
Format: Article
Language:English
Subjects:
alternative to antibiotics
Animal Feed - analysis
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics
Campylobacter
Campylobacter - drug effects
Campylobacter - physiology
chlortetracycline
diet
enterohemorrhagic Escherichia coli
Enzymes
Escherichia coli
feces
Feces - microbiology
genes
Hogs
hygiene
lysozyme
Microbiology
Muramidase - pharmacology
Pathogens
pigs
Salmonella
Salmonella - drug effects
Salmonella - physiology
Shiga-Toxigenic Escherichia coli - drug effects
Shiga-Toxigenic Escherichia coli - physiology
swine
Swine - growth & development
Swine - microbiology
Swine Diseases - drug therapy
Swine Diseases - microbiology
Swine Diseases - physiopathology
tiamulin
virulence
Zoonoses
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Summary:AIMS: The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. METHODS AND RESULTS: Two replicates of approximately 650 pigs each were weaned and randomly assigned to one of 24 pens in either a nursery room that had been fully disinfected or a nursery room left unclean. Pigs were randomly assigned to control diet (Control), control diet + antibiotics (Antibiotic; chlortetracycline and tiamulin), or control diet + lysozyme (Lysozyme; 100 mg kg⁻¹diet). Rectal swab samples were collected on day 0 and 28 of treatment, and enriched and cultured for Campylobacter spp. and shiga‐toxigenic Escherichia coli (STEC). Enrichments from rectal swab samples also were analysed for presence of enterohaemorrhagic E. coli (EHEC) virulence genes (hlyA, eae, stx1 and stx2). Room hygiene had little effect on day 28 results. Percentage of samples culture positive for Campylobacter spp. was lowest for lysozyme diets (P  0·1), but there was a tendency for fewer samples positive for stx1/stx2 in antibiotic or lysozyme diet groups (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12803