Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T

The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22days at two different cultivation temperatures (4 and 15°C). During the incubation p...

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Bibliographic Details
Published in:Marine pollution bulletin 2016-04, Vol.105 (1), p.125-130
Main Authors: Gentile, G., Bonsignore, M., Santisi, S., Catalfamo, M., Giuliano, L., Genovese, L., Yakimov, M.M., Denaro, R., Genovese, M., Cappello, S.
Format: Article
Language:English
Subjects:
4',6-diamidino-2-phenylindole
Bacteria
biodegradation
Bioremediation
DNA
gas chromatography
Hydrocarbonoclastic bacteria
ionization
Marine
Oil spill
oils
Oleispira antarctica
petroleum
Psychrophilic bacteria
staining
temperature
wastes
water pollution
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Summary:The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22days at two different cultivation temperatures (4 and 15°C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC–FID (gas chromatography–flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4′,6-diamidino-2-phenylindole) staining showed a linear increase during the first 18days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC–FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15°C rather than 4°C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4°C. •This study investigated biodegradation in cold marine environments.•Treatment of naval transportation wastes is essential.•Oleispira sp. is a potential candidate that plays a key role in the cleanup treatments of such wastes.
ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2016.02.041