1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish

Parkinson's disease (PD) is the most common age associated neurodegenerative disease, which has been extensively studied for its etiology and phenotype. PD has been widely studied in alternate model system such as rodents towards understanding the role of neurotoxin by inducing PD. This study i...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2016-05, Vol.16 (9), p.1407-1420
Main Authors: Sarath Babu, Nukala, Murthy, Ch Lakshmi N., Kakara, Sameera, Sharma, Rahul, Brahmendra Swamy, Cherukuvada V., Idris, Mohammed M.
Format: Article
Language:English
Subjects:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - administration & dosage
Animal proteomics
Animals
Behavior, Animal
Brain - metabolism
Danio rerio
Disease Models, Animal
Female
Gene Expression Profiling
Gene Expression Regulation
Gene Ontology
Genotype & phenotype
Humans
Injections, Intraperitoneal
Male
Markers
Molecular Sequence Annotation
MPTP
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Parkinson Disease, Secondary - chemically induced
Parkinson Disease, Secondary - genetics
Parkinson Disease, Secondary - metabolism
Parkinson Disease, Secondary - physiopathology
Parkinson's disease
Proteins
Proteome - genetics
Proteome - metabolism
Zebrafish
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:Parkinson's disease (PD) is the most common age associated neurodegenerative disease, which has been extensively studied for its etiology and phenotype. PD has been widely studied in alternate model system such as rodents towards understanding the role of neurotoxin by inducing PD. This study is aimed to understand the biomechanism of PD in zebrafish model system induced by 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP). The phenotype and role of various genes and proteins for Parkinsonism were tested and evaluated in this study using behavior, molecular and proteomic approaches. Zebrafish PD model induced by 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine showed a significant level of decrease in the movement with erratic swimming pattern and increased freezing bouts. CHCHD2, EEF2B, LRRK2, PARK7, PARK2, POLG, SNCGB and SYNB genes were differentially regulated at the transcript level in PD zebrafish. Similarly a total of 73 proteins were recognized as differentially expressed in the nervous system of zebrafish due to Parkinsonism based on quantitative proteomics approach. Proteins such as NEFL, MUNC13‐1, NAV2 and GAPVD1 were down regulated in the zebrafish brain for the PD phenotype, which were associated with the neurological pathways. This zebrafish based PD model can be used as a potential model system for screening prospective drug molecules for PD.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201500291