Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming

ABSTRACT Regulation of placental nutrient transport significantly affects fetal development and may modify intrauterine growth restriction (IUGR) and fetal programming. We hypothesized that placental nutrient transporters are differentially affected both by utero‐placental insufficiency and prenatal...

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Published in:Journal of cellular biochemistry 2016-07, Vol.117 (7), p.1594-1603
Main Authors: Nüsken, Eva, Gellhaus, Alexandra, Kühnel, Elisabeth, Swoboda, Isabelle, Wohlfarth, Maria, Vohlen, Christina, Schneider, Holm, Dötsch, Jörg, Nüsken, Kai-Dietrich
Format: Article
Language:English
Subjects:
Amino Acid Transport System A - metabolism
Amino Acid Transport Systems - metabolism
Animals
CD36 Antigens - metabolism
Connexins - metabolism
DEVELOPMENT
Fatty Acid Binding Protein 3
Fatty Acid-Binding Proteins - metabolism
Female
FETUS
Glucose Transporter Type 1 - metabolism
INTRAUTERINE GROWTH RESTRICTION
LIGATION
NUTRIENT TRANSPORTER
PLACENTA
Placenta - metabolism
Pregnancy
Rats
Rats, Wistar
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Summary:ABSTRACT Regulation of placental nutrient transport significantly affects fetal development and may modify intrauterine growth restriction (IUGR) and fetal programming. We hypothesized that placental nutrient transporters are differentially affected both by utero‐placental insufficiency and prenatal surgical stress. Pregnant rats underwent bilateral uterine artery and vein ligation (LIG), sham operation (SOP) or no operation (controls, C) on gestational day E19. Placentas were obtained by caesarean section 4 h (LIG, n=20 placentas; SOP, n=24; C, n=12), 24 h (LIG, n=28; SOP, n=20; C, n=12) and 72 h (LIG, n=20; SOP, n=20; C, n=24) after surgery. Gene and protein expression of placental nutrient transporters for fatty acids (h‐FABP, CD36), amino acids (SNAT1, SNAT2) and glucose (GLUT‐1, Connexin 26) were examined by qRT‐PCR, western blot and immunohistochemistry. Interestingly, the mean protein expression of h‐FABP was doubled in placentas of LIG and SOP animals 4, 24 (SOP significant) and 72 h (SOP significant) after surgery. CD36 protein was significantly increased in LIG after 72 h. SNAT1 and SNAT2 protein and gene expressions were significantly reduced in LIG and SOP after 24 h. Further significantly reduced proteins were GLUT‐1 in LIG (4 h, 72 h) and SOP (24 h), and Connexin 26 in LIG (72 h). In conclusion, placental nutrient transporters are differentially affected both by reduced blood flow and stress, probably modifying the already disturbed intrauterine milieu and contributing to IUGR and fetal programming. Increased fatty acid transport capacity may affect energy metabolism and could be a compensatory reaction with positive effects on brain development. J. Cell. Biochem. 117: 1594–1603, 2016. © 2015 Wiley Periodicals, Inc. We show that placental nutrient transporters are differentially affected both by utero‐placental insufficiency [induced by bilateral ligation (LIG) of the uterine vessels] and prenatal surgical stress [induced by sham operation (SOP)] in rats, which probably contributes to intrauterine growth restriction (IUGR) and fetal programming. The mean protein expressions of placental nutrient transporters for fatty acids (h‐FABP, CD36) were increased by LIG and SOP, amino acid transporter (SNAT1, SNAT2) protein and gene expressions as well as glucose transporter (GLUT‐1, Connexin 26) protein expressions were reduced. Increased fatty acid transport capacity may affect energy metabolism and could be a compensatory reaction with positive effe
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.25450