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Low Serum Vitamin B-12 Concentrations Are Prevalent in a Cohort of Pregnant Canadian Women
Among Canadian women of reproductive age, 5% and 20% have serum vitamin B-12 concentrations indicative of deficiency (271 nmol/L), and no women had elevated tHcy (>13 μmol/L). Maternal dietary vitamin B-12 intake during pregnancy was either weakly associated or not associated with maternal and co...
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| Published in: | The Journal of nutrition 2016-05, Vol.146 (5), p.1035-1042 |
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| container_title | The Journal of nutrition |
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| creator | Visentin, Carly E Masih, Shannon P Plumptre, Lesley Schroder, Theresa H Sohn, Kyoung-Jin Ly, Anna Lausman, Andrea Y Berger, Howard Croxford, Ruth Lamers, Yvonne Kim, Young-In O'Connor, Deborah L |
| description | Among Canadian women of reproductive age, 5% and 20% have serum vitamin B-12 concentrations indicative of deficiency (271 nmol/L), and no women had elevated tHcy (>13 μmol/L). Maternal dietary vitamin B-12 intake during pregnancy was either weakly associated or not associated with maternal and cord blood vitamin B-12 (r(2) = 0.17-0.24, P < 0.0008), tHcy (P = NS) and MMA (r(2) = 0.05-0.11, P < 0.001). Fetal genetic polymorphisms were not associated with cord blood concentrations of vitamin B-12 and its biomarkers.
Deficient and marginal serum vitamin B-12 concentrations are prevalent in Canadian pregnant women with the use of traditional cutoffs, despite supplement use. Given the growing interest among women to adhere to a vegetarian diet that may be lower in vitamin B-12, and vitamin B-12's importance in pregnancy, the functional ramifications of these observations need to be elucidated. This trial was registered at clinicaltrials.gov as NCT02244684. |
| doi_str_mv | 10.3945/jn.115.226845 |
| format | article |
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This prospective analysis from the PREFORM (PREnatal FOlic acid exposuRe on DNA Methylation in the newborn infant) study investigated 1) vitamin B-12 status in a cohort of Canadian pregnant women and their newborns, 2) the association of maternal dietary vitamin B-12 intake with maternal and cord blood concentrations of vitamin B-12 and its biomarkers, and 3) the association of fetal genetic polymorphisms with cord blood concentrations of vitamin B-12 and its biomarkers.
In pregnant Canadian women (n = 368; mean ± SD age: 32 ± 5 y), vitamin B-12 intakes were assessed in early (0-16 wk) and mid- to late (23-37 wk) pregnancy. Serum vitamin B-12 and plasma total homocysteine (tHcy) and methylmalonic acid (MMA) in maternal blood at 12-16 wk of pregnancy and at delivery (28-42 wk) and in cord blood were measured and compared by using regression analyses. The associations of 28 fetal genetic variants in vitamin B-12 metabolism and cord blood vitamin B-12, tHcy, and MMA concentrations were assessed by using regression analysis, with adjustment for multiple testing.
A total of 17% and 38% of women had deficient and 35% and 43% had marginal serum vitamin B-12 concentrations at 12-16 wk of pregnancy and at delivery, respectively. Only 1.9-5.3% had elevated MMA (>271 nmol/L), and no women had elevated tHcy (>13 μmol/L). Maternal dietary vitamin B-12 intake during pregnancy was either weakly associated or not associated with maternal and cord blood vitamin B-12 (r(2) = 0.17-0.24, P < 0.0008), tHcy (P = NS) and MMA (r(2) = 0.05-0.11, P < 0.001). Fetal genetic polymorphisms were not associated with cord blood concentrations of vitamin B-12 and its biomarkers.
Deficient and marginal serum vitamin B-12 concentrations are prevalent in Canadian pregnant women with the use of traditional cutoffs, despite supplement use. Given the growing interest among women to adhere to a vegetarian diet that may be lower in vitamin B-12, and vitamin B-12's importance in pregnancy, the functional ramifications of these observations need to be elucidated. This trial was registered at clinicaltrials.gov as NCT02244684.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.3945/jn.115.226845</identifier><identifier>PMID: 27075906</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Canada - epidemiology ; Diet ; Dietary Supplements ; DNA Methylation ; Female ; Fetal Blood - metabolism ; Fetus ; Folic Acid - blood ; Homocysteine - blood ; Humans ; Infant, Newborn ; Methylmalonic Acid - blood ; Polymorphism, Genetic ; Pregnancy ; Pregnancy Complications - epidemiology ; Prevalence ; Prospective Studies ; Vitamin B 12 - administration & dosage ; Vitamin B 12 - blood ; Vitamin B 12 Deficiency - epidemiology ; Vitamin B Complex - administration & dosage ; Vitamin B Complex - blood</subject><ispartof>The Journal of nutrition, 2016-05, Vol.146 (5), p.1035-1042</ispartof><rights>2016 American Society for Nutrition.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-ecd44b8d28001cf9cd15f79abbcc09942bfe6d6ba643fccdaf7b027b4d622a813</citedby><cites>FETCH-LOGICAL-c332t-ecd44b8d28001cf9cd15f79abbcc09942bfe6d6ba643fccdaf7b027b4d622a813</cites><orcidid>0000-0001-6331-091X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27075906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Visentin, Carly E</creatorcontrib><creatorcontrib>Masih, Shannon P</creatorcontrib><creatorcontrib>Plumptre, Lesley</creatorcontrib><creatorcontrib>Schroder, Theresa H</creatorcontrib><creatorcontrib>Sohn, Kyoung-Jin</creatorcontrib><creatorcontrib>Ly, Anna</creatorcontrib><creatorcontrib>Lausman, Andrea Y</creatorcontrib><creatorcontrib>Berger, Howard</creatorcontrib><creatorcontrib>Croxford, Ruth</creatorcontrib><creatorcontrib>Lamers, Yvonne</creatorcontrib><creatorcontrib>Kim, Young-In</creatorcontrib><creatorcontrib>O'Connor, Deborah L</creatorcontrib><title>Low Serum Vitamin B-12 Concentrations Are Prevalent in a Cohort of Pregnant Canadian Women</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Among Canadian women of reproductive age, 5% and 20% have serum vitamin B-12 concentrations indicative of deficiency (<148 pmol/L) and marginal status (148-220 pmol/L), respectively. Given the association between suboptimal vitamin B-12 and adverse pregnancy outcomes, an understanding of vitamin B-12 status during pregnancy, and factors that influence it, is required.
This prospective analysis from the PREFORM (PREnatal FOlic acid exposuRe on DNA Methylation in the newborn infant) study investigated 1) vitamin B-12 status in a cohort of Canadian pregnant women and their newborns, 2) the association of maternal dietary vitamin B-12 intake with maternal and cord blood concentrations of vitamin B-12 and its biomarkers, and 3) the association of fetal genetic polymorphisms with cord blood concentrations of vitamin B-12 and its biomarkers.
In pregnant Canadian women (n = 368; mean ± SD age: 32 ± 5 y), vitamin B-12 intakes were assessed in early (0-16 wk) and mid- to late (23-37 wk) pregnancy. Serum vitamin B-12 and plasma total homocysteine (tHcy) and methylmalonic acid (MMA) in maternal blood at 12-16 wk of pregnancy and at delivery (28-42 wk) and in cord blood were measured and compared by using regression analyses. The associations of 28 fetal genetic variants in vitamin B-12 metabolism and cord blood vitamin B-12, tHcy, and MMA concentrations were assessed by using regression analysis, with adjustment for multiple testing.
A total of 17% and 38% of women had deficient and 35% and 43% had marginal serum vitamin B-12 concentrations at 12-16 wk of pregnancy and at delivery, respectively. Only 1.9-5.3% had elevated MMA (>271 nmol/L), and no women had elevated tHcy (>13 μmol/L). Maternal dietary vitamin B-12 intake during pregnancy was either weakly associated or not associated with maternal and cord blood vitamin B-12 (r(2) = 0.17-0.24, P < 0.0008), tHcy (P = NS) and MMA (r(2) = 0.05-0.11, P < 0.001). Fetal genetic polymorphisms were not associated with cord blood concentrations of vitamin B-12 and its biomarkers.
Deficient and marginal serum vitamin B-12 concentrations are prevalent in Canadian pregnant women with the use of traditional cutoffs, despite supplement use. Given the growing interest among women to adhere to a vegetarian diet that may be lower in vitamin B-12, and vitamin B-12's importance in pregnancy, the functional ramifications of these observations need to be elucidated. This trial was registered at clinicaltrials.gov as NCT02244684.</description><subject>Adult</subject><subject>Canada - epidemiology</subject><subject>Diet</subject><subject>Dietary Supplements</subject><subject>DNA Methylation</subject><subject>Female</subject><subject>Fetal Blood - metabolism</subject><subject>Fetus</subject><subject>Folic Acid - blood</subject><subject>Homocysteine - blood</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Methylmalonic Acid - blood</subject><subject>Polymorphism, Genetic</subject><subject>Pregnancy</subject><subject>Pregnancy Complications - epidemiology</subject><subject>Prevalence</subject><subject>Prospective Studies</subject><subject>Vitamin B 12 - administration & dosage</subject><subject>Vitamin B 12 - blood</subject><subject>Vitamin B 12 Deficiency - epidemiology</subject><subject>Vitamin B Complex - administration & dosage</subject><subject>Vitamin B Complex - blood</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLxDAUhYMozji6dCtZuumYpGnaLMfiCwYUfIGbkKd2aJMxaRX_vR1mdHXh3I_D4QPgFKN5zmlxsfJzjIs5IayixR6Y4oLijGGE9sEUIUKyHDM2AUcprRBCmPLqEExIicqCIzYFb8vwDR9tHDr40vSyazy8zDCBdfDa-j7Kvgk-wUW08CHaL9mOIRwhORIfIfYwuM3j3csxr6WXppEevobO-mNw4GSb7MnuzsDz9dVTfZst72_u6sUy03lO-sxqQ6mqDKnGedpxbXDhSi6V0hpxTolylhmmJKO509pIVypESkUNI0RWOJ-B823vOobPwaZedE3Stm2lt2FIApdViSrOSjSi2RbVMaQUrRPr2HQy_giMxEanWHkx6hRbnSN_tqseVGfNP_3nL_8FnKtwJA</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Visentin, Carly E</creator><creator>Masih, Shannon P</creator><creator>Plumptre, Lesley</creator><creator>Schroder, Theresa H</creator><creator>Sohn, Kyoung-Jin</creator><creator>Ly, Anna</creator><creator>Lausman, Andrea Y</creator><creator>Berger, Howard</creator><creator>Croxford, Ruth</creator><creator>Lamers, Yvonne</creator><creator>Kim, Young-In</creator><creator>O'Connor, Deborah L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6331-091X</orcidid></search><sort><creationdate>201605</creationdate><title>Low Serum Vitamin B-12 Concentrations Are Prevalent in a Cohort of Pregnant Canadian Women</title><author>Visentin, Carly E ; Masih, Shannon P ; Plumptre, Lesley ; Schroder, Theresa H ; Sohn, Kyoung-Jin ; Ly, Anna ; Lausman, Andrea Y ; Berger, Howard ; Croxford, Ruth ; Lamers, Yvonne ; Kim, Young-In ; O'Connor, Deborah L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-ecd44b8d28001cf9cd15f79abbcc09942bfe6d6ba643fccdaf7b027b4d622a813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Canada - epidemiology</topic><topic>Diet</topic><topic>Dietary Supplements</topic><topic>DNA Methylation</topic><topic>Female</topic><topic>Fetal Blood - metabolism</topic><topic>Fetus</topic><topic>Folic Acid - blood</topic><topic>Homocysteine - blood</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Methylmalonic Acid - blood</topic><topic>Polymorphism, Genetic</topic><topic>Pregnancy</topic><topic>Pregnancy Complications - epidemiology</topic><topic>Prevalence</topic><topic>Prospective Studies</topic><topic>Vitamin B 12 - administration & dosage</topic><topic>Vitamin B 12 - blood</topic><topic>Vitamin B 12 Deficiency - epidemiology</topic><topic>Vitamin B Complex - administration & dosage</topic><topic>Vitamin B Complex - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Visentin, Carly E</creatorcontrib><creatorcontrib>Masih, Shannon P</creatorcontrib><creatorcontrib>Plumptre, Lesley</creatorcontrib><creatorcontrib>Schroder, Theresa H</creatorcontrib><creatorcontrib>Sohn, Kyoung-Jin</creatorcontrib><creatorcontrib>Ly, Anna</creatorcontrib><creatorcontrib>Lausman, Andrea Y</creatorcontrib><creatorcontrib>Berger, Howard</creatorcontrib><creatorcontrib>Croxford, Ruth</creatorcontrib><creatorcontrib>Lamers, Yvonne</creatorcontrib><creatorcontrib>Kim, Young-In</creatorcontrib><creatorcontrib>O'Connor, Deborah L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Visentin, Carly E</au><au>Masih, Shannon P</au><au>Plumptre, Lesley</au><au>Schroder, Theresa H</au><au>Sohn, Kyoung-Jin</au><au>Ly, Anna</au><au>Lausman, Andrea Y</au><au>Berger, Howard</au><au>Croxford, Ruth</au><au>Lamers, Yvonne</au><au>Kim, Young-In</au><au>O'Connor, Deborah L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low Serum Vitamin B-12 Concentrations Are Prevalent in a Cohort of Pregnant Canadian Women</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>2016-05</date><risdate>2016</risdate><volume>146</volume><issue>5</issue><spage>1035</spage><epage>1042</epage><pages>1035-1042</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Among Canadian women of reproductive age, 5% and 20% have serum vitamin B-12 concentrations indicative of deficiency (<148 pmol/L) and marginal status (148-220 pmol/L), respectively. Given the association between suboptimal vitamin B-12 and adverse pregnancy outcomes, an understanding of vitamin B-12 status during pregnancy, and factors that influence it, is required.
This prospective analysis from the PREFORM (PREnatal FOlic acid exposuRe on DNA Methylation in the newborn infant) study investigated 1) vitamin B-12 status in a cohort of Canadian pregnant women and their newborns, 2) the association of maternal dietary vitamin B-12 intake with maternal and cord blood concentrations of vitamin B-12 and its biomarkers, and 3) the association of fetal genetic polymorphisms with cord blood concentrations of vitamin B-12 and its biomarkers.
In pregnant Canadian women (n = 368; mean ± SD age: 32 ± 5 y), vitamin B-12 intakes were assessed in early (0-16 wk) and mid- to late (23-37 wk) pregnancy. Serum vitamin B-12 and plasma total homocysteine (tHcy) and methylmalonic acid (MMA) in maternal blood at 12-16 wk of pregnancy and at delivery (28-42 wk) and in cord blood were measured and compared by using regression analyses. The associations of 28 fetal genetic variants in vitamin B-12 metabolism and cord blood vitamin B-12, tHcy, and MMA concentrations were assessed by using regression analysis, with adjustment for multiple testing.
A total of 17% and 38% of women had deficient and 35% and 43% had marginal serum vitamin B-12 concentrations at 12-16 wk of pregnancy and at delivery, respectively. Only 1.9-5.3% had elevated MMA (>271 nmol/L), and no women had elevated tHcy (>13 μmol/L). Maternal dietary vitamin B-12 intake during pregnancy was either weakly associated or not associated with maternal and cord blood vitamin B-12 (r(2) = 0.17-0.24, P < 0.0008), tHcy (P = NS) and MMA (r(2) = 0.05-0.11, P < 0.001). Fetal genetic polymorphisms were not associated with cord blood concentrations of vitamin B-12 and its biomarkers.
Deficient and marginal serum vitamin B-12 concentrations are prevalent in Canadian pregnant women with the use of traditional cutoffs, despite supplement use. Given the growing interest among women to adhere to a vegetarian diet that may be lower in vitamin B-12, and vitamin B-12's importance in pregnancy, the functional ramifications of these observations need to be elucidated. This trial was registered at clinicaltrials.gov as NCT02244684.</abstract><cop>United States</cop><pmid>27075906</pmid><doi>10.3945/jn.115.226845</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6331-091X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of nutrition, 2016-05, Vol.146 (5), p.1035-1042 |
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| source | Alma/SFX Local Collection |
| subjects | Adult Canada - epidemiology Diet Dietary Supplements DNA Methylation Female Fetal Blood - metabolism Fetus Folic Acid - blood Homocysteine - blood Humans Infant, Newborn Methylmalonic Acid - blood Polymorphism, Genetic Pregnancy Pregnancy Complications - epidemiology Prevalence Prospective Studies Vitamin B 12 - administration & dosage Vitamin B 12 - blood Vitamin B 12 Deficiency - epidemiology Vitamin B Complex - administration & dosage Vitamin B Complex - blood |
| title | Low Serum Vitamin B-12 Concentrations Are Prevalent in a Cohort of Pregnant Canadian Women |
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