Growth Factor Treatment Enhances Vestibular Hair Cell Renewal and Results in Improved Vestibular Function

The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor α (TGFα), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), where...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-05, Vol.98 (10), p.5886-5891
Main Authors: Kopke, Richard D., Jackson, Ronald L., Li, Geming, Rasmussen, Mark D., Hoffer, Michael E., Frenz, Dorothy A., Costello, Michael, Schultheiss, Peter, Van De Water, Thomas R.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cell growth
Cell nucleus
Cells
Electron micrographs
Gentamicins - administration & dosage
Growth rate
Growth Substances - pharmacology
Guinea Pigs
Hair
Hair cells
Hair Cells, Vestibular - drug effects
Hair Cells, Vestibular - physiology
Hair Cells, Vestibular - ultrastructure
Low speed
Material concentration
Microscopy, Electron
Neurons
Neuroscience
Pharmacology
Vestibular hair cells
Vestibular labyrinth
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Summary:The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor α (TGFα), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), whereas GF II contained those three factors and brain-derived neurotrophic factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.101120898