Development and Validation of a Stability-Indicating Micellar Liquid Chromatographic Method for the Determination of Timolol Maleate in the Presence of Its Degradation Products

A stability-indicating micellar liquid chromatographic (MLC) method was developed and validated for the quantitative determination of timolol maleate (TM) in the presence of its degradation products resulting from accelerated degradation in a run time not more than 8 min. TM was subjected to stress...

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Bibliographic Details
Published in:Journal of chromatographic science 2015-04, Vol.53 (4), p.503-510
Main Authors: Rizk, Mohamed S., Merey, Hanan A., Tawakkol, Shereen M., Sweilam, Mona N.
Format: Article
Language:English
Subjects:
Chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Degradation
Drug Stability
Guidelines
Hydrolysis
Linear Models
Liquids
Maleates
Ophthalmic Solutions - analysis
Ophthalmic Solutions - chemistry
Phenyls
Reproducibility of Results
Sensitivity and Specificity
Timolol - analysis
Timolol - chemistry
Triethylamine
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Summary:A stability-indicating micellar liquid chromatographic (MLC) method was developed and validated for the quantitative determination of timolol maleate (TM) in the presence of its degradation products resulting from accelerated degradation in a run time not more than 8 min. TM was subjected to stress conditions of hydrolysis (including alkaline, acidic and thermal hydrolysis) and oxidation. An isocratic, rapid and mobile phase saving the micellar LC method was developed with a BioBasic phenyl column (150 × 1.0 mm, 5 µm particle size) and a micellar mobile phase composed of 0.1 M sodium dodecyl sulfate, 10% of 1-propanol and 0.1% of triethylamine in 0.035 M ortho-phosphoric acid. The flow rate of the mobile phase was 0.1 mL/min. UV detection was adjusted at 298 nm and performed at room temperature. The method has been validated according to the International Conference on Harmonisation guidelines. The method is successfully applied for the determination of TM in bulk powder and pharmaceutical dosage form.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmu075